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Main Title Hydroxyl Free Radical Mediated Formation of 8-Hydroxyguanine in Isolated DNA (Journal Version).
Author Floyd, R. A. ; West, M. S. ; Eneff, K. L. ; Hogsett, W. E. ; Tingey, D. T. ;
CORP Author Oklahoma Medical Research Foundation, Oklahoma City.;Corvallis Environmental Research Lab., OR.
Publisher c1988
Year Published 1988
Report Number EPA-R-812710; EPA/600/J-88/225;
Stock Number PB89-144562
Additional Subjects Deoxyribonucleic acids ; Free radicals ; Guanines ; Ultraviolet radiation ; Hydrogen peroxide ; Ethyl alcohol ; Thioureas ; Salicylates ; Glutathione ; Thymus gland ; Sugar alcohols ; Scavenging ; Reprints ; Hydroxyguanine ; Biological radiation effects ; Hydroxyl radicals ; DNA ; Cows ; Mannitol
Library Call Number Additional Info Location Last
NTIS  PB89-144562 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Formation of 8-hydroxyguanine within calf thymus DNA has been studied after exposure to uv-H2O2 as a hydroxyl free radical generating system. Using high-pressure liquid chromatography with electrochemical detection, the amount of 8-hydroxy-2-deoxyguanosine (8-OHdG) was measured. The 8-OHdG content of uv-exposed DNA increased linearly with increasing H2O2 levels up to 0.03%, above which the rate of increase was less than linear. All hydroxyl free radical scavengers studied (mannitol, ethanol, thiorea, and salicylate), if present in the system when DNA was exposed to uv-H2O2, caused a decrease in the amount of 8-OHdG formed. Thiourea when incubated with damaged DNA caused a loss of 8-OHdG when it was an integral part of DNA. In contrast, thiorea did not react with the nucleoside free in solution. Reduced glutathione did not cause a decrease of 8-OHdG, either when it was an integral part of DNA, or as the free nucleoside in solution. (Copyright (c) 1988 by Academic Press, Inc.)