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Main Title Study of the Hepatic Effects of Di-(2-Ethylhexyl) Adipate in the Mouse and Rat with Appendices and Cover Letter dated 11/17/89.
CORP Author Chemical Manufacturers Association, Washington, DC.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Publisher 27 Dec 1989
Year Published 1989
Report Number FYI-OTS-0190-0731;
Stock Number OTS-0000731
Additional Subjects Rats ; Mice ; Body weight ; Food intake ; Triglycerides ; Alanine aminotransferase ; Alkaline phosphatase ; Liver ; Spleen ; Assays ; Peroxisomes ; Di-(2-ethyhexyl)adipate ; Fischer 344 strain rats ; B6C3F1 strain mice ; Liver samples ; DNA concentration ; Total cholesterol ; Total bilirubin
Library Call Number Additional Info Location Last
NTIS  OTS-0000731 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 302p
Di-(2-ethylhexyl) adipate (DEHA) was fed to groups of twenty female Fischer 344 strain rats or thirty B6C3F1 strain mice at dietary levels of 0(control), 0.012, 0.12, 1.2 or 2.5% for up to 21 days. In both species there was also a recovery group of ten rats and twenty mice fed 2.5% DEHA for 21 days followed by 14 days on control diet, matched with a second control group fed the basal diet for 35 days. Body weights and food intakes were monitored prior to and throughout the treatment and recovery periods. After 3, 7 or 21 days of treatment five animals of both species from each dietary level were given at intraperitoneal dose of ((sup 3) H-methyl) thymidine and killed one hour later for the collection of liver samples for the assay of DNA concentration and ((sup 3)H) thymidine uptake. At the 21 day examination additional rats and mice were killed and blood collected for the determination of serum triglycerides, total cholesterol, total bilirubin, alanine aminotransferase and alkaline phosphatase. The livers, kidney and spleens were weighed and preserved for histological examination. In addition, samples of liver were processed for electron microscopic examination of peroxisomes and for histochemical demonstration of neutral fat.