||Feasibility of applying field ionization mass spectroscopy to pesticide research /
Dyer, R. L. ;
Heck, H. d'A. ;
Scott, A. C. ;
||Stanford Research Inst., Menlo Park, Calif.;Health Effects Research Lab., Research Triangle Park, N.C.
|| U.S. Environmental Protection Agency, Health Effects Research Laboratory,
||PB 262 241
Chemical analysis ;
Mass spectroscopy ;
Nitro compounds ;
Isotopic labeling ;
Gas chromatography ;
Error analysis ;
Thin layer chromatography ;
Biological extracts ;
Methyl parathion ;
Field ionization mass spectroscopy ;
||Research Triangle Park Library/RTP, NC
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||ix, 77 p. ; 28 cm.
An isotope dilution methodology was developed for analysis of an insecticide, parathion, and a herbicide, trifluralin, isolated from rat tissues and excreta. Sample cleanup was facilitated by use of high-pressure gel permeation chromatography in conjunction with thin layer chromatography and reversed-phase high pressure liquid chromatography. Isotope ratio measurements were performed using multilabeled stable isotopic carriers and nonfragmenting field ionization mass spectrometry. Parathion and trifluralin were administered intraperitoneally and/or orally at the sub-mg/kg level, and the unchanged materials assayed in tissues and excreta at the ppb level. The technique was also applied to the determination of parathion and methyl parathion concentrations in aerosols. The biological implications of the results of the animal experiments are discussed.