||Bioaugmentation with Burkholderia cepacia PR1301 for in situ bioremediation of trichloroethylene contaminated water /
McCarty, P. L. ;
Hopkins, G. D. ;
Munakata-Marr, J. ;
Matheson, V. G. ;
Dolan, M. E.
||Stanford Univ., CA. Western Region Hazardous Substance Research Center. ;University of West Florida, Pensacola. ;Michigan State Univ., East Lansing. Center for Microbial Ecology.;National Health and Environmental Effects Research Lab., Gulf Breeze, LA. Gulf Ecology Div.
|| U.S. Environmental Protection Agency, Research and Development, National Health and Environmental Effects Research Laboratory,
||EPA/600-S-98-001; EPA-R-822029; PB98141542
Water--Purification--Biological treatment ;
In situ bioremediation ;
Water treatment ;
In situ processing ;
Aerobic conditions ;
Ground water ;
Water pollution control ;
Biochemical reaction kinetics ;
Laboratory tests ;
Burkholderia cepacia PR1(sub 301) ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||11 pages : illustrations ; 28 cm.
A pilot field study was conducted at the Moffett Federal Airfield, Mountain View, CA, to determine whether effective in situ aerobic cometabolic biodegradation of trichloroethylene (TCE) could be accomplished through bioaugmentation with a genetically modified strain of Burkholderia cepacia G4 (G4) together with feeding of lactate to serve as an energy and growth substrate for the organism. Strain G4 is highly effective at TCE cometabolism but requires either phenol or toluene to induce oxygenase enzyme activity. A strain of G4 was developed through NTG mutagenesis that constitutively expresses the oxygenase so that no inducer need be added. The modified strain, B. cepacia PR1(sub 301) (PR1 (sub 301)), can degrade TCE effectively while growing on simple substrates such as lactate. Strain-specific molecular probes were developed for monitoring the presence and movement of PR1 (sub 301) and were based upon rep-PCR analysis.
Caption title. Shipping list no.: 98-0247-P. "April 1998." Includes bibliographical references (page 11). "EPA/600-S-98-001."