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Main Title Increased 8-Hydroxyguanine Content of Chloroplast DNA from Ozone-Treated Plants.
Author Floyd, R. A. ; West, M. S. ; Hogsett, W. E. ; Tingey, D. T. ;
CORP Author Oklahoma Medical Research Foundation, Oklahoma City.;Corvallis Environmental Research Lab., OR.
Publisher c1989
Year Published 1989
Report Number EPA-CR-812710-01-0; EPA/600/J-89/303;
Stock Number PB90-198664
Additional Subjects Deoxyribonucleic acids ; Plant genetics ; Ozone ; Chloroplasts ; Free radicals ; Tables(Data) ; Beans ; Peas ; Reprints ; Air pollution effects(Plants) ; Hydroxy guanine ; DNA damage ; Phaseolus vulgaris ; Pisum sativum
Library Call Number Additional Info Location Last
NTIS  PB90-198664 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
The mechanism of ozone-mediated plant injury is not known but has been postulated to involve oxygen free radicals. Hydroxyl free radicals react with DNA causing formation of many products, one of which is 8-hydroxyguanine. By using high performance liquid chromatography with electrochemical detection, the 8-hydroxy-2'-deoxyguanosine (8-OHdG) content of a DNA enzymatic digest can be sensitively quantitated. Beans and peas were treated with an ozone regime that caused acute injury. Chloroplast DNA was obtained from plants harvested either immediately after ozone treatment or 24 hours later. Ozone-exposed plants in general had nearly two-fold higher levels of 8-OHdG as compared to control plants. In vitro treatment of DNA in buffer solution with ozone did not cause formation of 8-OHdG in DNA, even though ozone did react directly with the macromolecule per se. Exposure of isolated illuminated chloroplasts to ozone caused nearly a seven-fold increase in the amount of 8-OHdG in the chloroplast DNA as compared to none-oxone-exposed chloroplasts. The results suggest that ozone exposure to plants causes formation of enhanced levels of oxygen free radicals, thus mediating formation of 8-OHdG in chloroplast DNA. The reaction of ozone with DNA per se did not cause formation of 8-OHdG.