Record Display for the EPA National Library Catalog


Main Title Restriction Map of 'Rachiplusia ou' and 'Rachiplusia ou-Autographa californica' Baculovirus Recombinants.
Author Smith, Gale E. ; Summers, Max D. ;
CORP Author Texas A and M Univ., College Station. Dept. of Entomology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1980
Report Number EPA-R-805232-01; EPA-600/J-80-253;
Stock Number PB81-192056
Additional Subjects Viruses ; Deoxyribonucleic acid ; Genes ; Molecular biology ; Microorganisms ; Chromosomes ; Cells(Biology) ; Peptides ; Reprints ; Autographa californica ; Rachiplusia ou
Library Call Number Additional Info Location Last
NTIS  PB81-192056 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The restriction sites of Rachiplusia ou nuclear polyhedrosis virus (RoMNPV) DNA were mapped for the endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI. Of the 60 DNA restriction sites of RoMNPV, 35 mapped in similar positions as compared to the restriction sites of Autographa californica nuclear polyhedrosis virus (AcMNPV) DNA. Two plaque-purified viruses, obtained from randomly picked plaques of a wild-type isolate of RoMNPV, were recombinants of RoMNPV and AcMNPV. The recombinants were shown to have RoMNPV and AcMNPV restriction fragments as well as structural polypeptides from each parental virus. Both recombinant viruses had a major RoMNPV capsid protein but were occluded in the AcMNPV polyhedrin protein.