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Main Title Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction.
Author Delnomdedieu, M. ; Basti, M. M. ; Otvos, J. D. ; Thomas, D. J. ;
CORP Author North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology. ;North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch.
Publisher cOct 93
Year Published 1993
Report Number EPA-R-817643; EPA/600/J-94/532;
Stock Number PB95-148946
Additional Subjects Glutathione ; Arsenites ; Chemical models ; Thiols ; Nuclear magnetic resonance spectroscopy ; Binding sites ; Arsenates ; Reduction(Chemistry) ; Carbon 13 ; Reprints ; Succinic acid/meso-2,3-dimercapto
Library Call Number Additional Info Location Last
NTIS  PB95-148946 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
The interactions of arsenate and arsenite with meso-2,3-dimercaptosuccinic acid (DMSA) have been characterized using carbon-13 nuclear magnetic resonance. These studies show that DMSA reduces arsenate to arsenite and complexes arsenite. Monitoring the carbon-13 signals of complexed DMSA and liberated glutathione shows that DMSA readily extracts arsenite from a glutathione(sub 3)-arsenite complex, proving the affinity of arsenite for dithiols is greater than that for monothiols. Competition between DMSA (vicinal thiols) and dithioerythritol (1,4-dimercapto-2,3-butanediol) for binding of arsenite indicates that the binding affinity is inversely related to the distance between the two thiol groups. On the basis of these findings, a model for the interaction of arsenic with mono- and dithiol-containing molecules is proposed. (Copyright (c) 1993 by the American Chemical Society.)