Record Display for the EPA National Library Catalog


Main Title Induction of 6-Thioguanine Resistance in Synthronized Human Fibroblast Cells Treated with Methyl Methanesulfonate, N-Acetoxy-2-Acethylaminofluorene and N-Methyl-N'-Nitro-N-Nitrosoguanidine.
Author Huang, Shiu L. ; Huang, Shu-Mei S. ; Casperson, Charlotte ; Waters, Michael D. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-600/J-81-181;
Stock Number PB83-209726
Additional Subjects Humans ; Nitrogen organic compounds ; Sulfur organic compounds ; Deoxyribonucleic acids ; Reprints ; Thioguanine ; Fibroblasts ; Methane sulfonic acid/(methyl-ester) ; Fluorene/N-acetoxy-acetylamino ; Guanidine/N-methyl-N-nitro-N-nitroso ; Chemical treatments
Library Call Number Additional Info Location Last
NTIS  PB83-209726 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Chemical induction of 6-thioguanine resistance was studied in synchronized human fibroblast cells. Cells initially grown in a medium lacking arginine and glutamine for 24 h ceased DNA synthesis and failed to enter the S phase. After introduction of complete medium, the cells progressed to the S phase after 16 h. DNA synthesis peaked 20 h after removal of nutrient stress and declined. Mutations were induced in S-phase cells by methyl methanesulfonate (MMS), N-acetoxy-2-acetylaminofluorene (NA-AAF) and N-methyl-N nitro-N-nitrosoguanidine (MNNG). Chemical treatments resulted in an increase in the absolute number of mutant colonies and in a dose-dependent mutation frequency.