Record Display for the EPA National Library Catalog


Main Title Induction of Micronuclei in Cultured Human Bronchial Epithelial Cells by Direct-Acting Carcinogens.
Author Andrews, P. G. ; Owens, S. E. ; Siegfried, J. M. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Pittsburgh Univ., PA. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA-68-02-4456; EPA/600/J-90/479;
Stock Number PB91-182113
Additional Subjects Micronucleus tests ; Bronchi ; Carcinogens ; Epithelium ; Cultured cells ; Methylnitronitrosoguanidine ; Cell survival ; Cytokinetics ; Fluorescence microscopy ; Reprints ; Dihydroxyepoxytetrahydrobenzo(a)pyrenes
Library Call Number Additional Info Location Last
NTIS  PB91-182113 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The sensitivity of human bronchial epithelial cells to induction of micronuclei was determined in cultures derived from different donors. Two direct-acting carcinogens, (+ or -)-7,8 -dihydroxy-9,10-epoxy-7,8,9,10- tetrahydrobenzo (a)- pyrene (BPDE) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were used to induce micronuclei. Both agents increased the incidence of micronuclei in a concentration-dependent fashion, even at concentrations which did not produce cytotoxicity. Cytokinesis was blocked with cytochalasin B so that micronuclei were counted only in binucleate cells, thereby decreasing the total number of cells that need to be examined and also eliminating variations due to possible differences in cell growth rates. The results demonstrate the potential usefulness of the micronucleus assay as a sensitive measure of genetic damage in human epithelial cells. (Copyright (c) 1990 Pergamon Press plc.)