Record Display for the EPA National Library Catalog


Main Title Cytotoxicity of Chemical Carcinogens Towards Human Bronchial Epithelial Cells Evaluated in a Clonal Assay.
Author Siegfried, J. M. ; Nesnow, S. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1984
Year Published 1984
Report Number EPA-68-02-4031; EPA/600/J-84/412;
Stock Number PB89-103618
Additional Subjects Toxicology ; Carcinogens ; Bronchii ; Clone cells ; Toxicity ; Cell lines ; Epithelium ; Culture media ; Methodology ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB89-103618 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a clonal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and clonal growth. The clonal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported clonal growth of the human cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene, large variations in survival were found between cultures derived from different individuals. (Copyright (c) IRL Press Ltd.)