|Gradient Liquid Chromatography/Mass Spectrometry Using Microbore Columns and a Moving Belt Interface.
Hayes, M. J. ;
Schwartz, H. E. ;
Vouros, P. ;
Karger, B. L. ;
|Environmental Research Lab., Athens, GA. Office of Research and Development. ;Northeastern Univ., Boston, MA. Barnett Inst. of Chemical Analysis and Materials Science.
Mass spectroscopy ;
Column packings ;
Qualitative analysis ;
Belt conveyors ;
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High-performance gradient microbore LC/MS is demonstrated by use of spray deposition onto a moving belt surface. A simplified nebulizer design is shown to provide convenient operation and high transfer efficiency over a wide range of flow and solvent conditions. It is shown further that the extracolumn variance of the LC/MS system is sufficiently small to allow the use of relatively short 5 micrometers and 7.5 micrometers reversed-phase microbore columns without significant loss in resolution. Relatively high microbore flow rates (about 100 microL/min) are possible for fast analysis and sensitivity. The LC/MS technique is applied to the analysis of phenols in a aqueous coal gasification sample. Detection limits for full scan LC/EI-MS are found to be about 6 ng for some representative phenols. (Copyright (c) 1984 American Chemical Society.)