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Main Title Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version).
Author Schupp, D. G. ; Januschka, M. M. ; Sherlock, L. A. F. ; Stibbs, H. H. ; Meyer, E. A. ;
CORP Author Minnesota Univ., Minneapolis. ;Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology. ;Washington Univ., Seattle. School of Public Health and Community Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/116;
Stock Number PB89-109359
Additional Subjects Giardiasis ; Parasitic intestinal diseases ; Protozoa ; Giardia ; Cultured cells ; Cysts ; Immunohistochemistry ; Morphology ; Electron microscopy ; Laboratory animals ; Life cycle ; Infectivity ; Gerbils ; Reprints ; Trophozoites
Library Call Number Additional Info Location Last
NTIS  PB89-109359 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The purpose of the research was to document the formation of viable Giardia cysts in vitro. Viability staining, using fluorogenic dyes that required metabolic conversion for detection, and immunocytochemistry at the light microscopic level, provided information on viability as well as the identification of antigens that were similar to those found with in vitro formed cysts. Analysis of cysts formed in vivo and in vitro showed similar morphological appearances by both light and electron microscopy. Cysts formed in vitro were capable of establishing infections in both the mouse and gerbil models for giardiasis. Trophozoites recovered from mice experimentally infected with cysts produced in vitro could be maintained in culture and induced a second time to form cysts in vitro. The model, for the production of viable Giardia cysts in vitro, should facilitate research on controlling the complete life-cycle of Giardia outside an animal host. (Copyright (c) 1988 by the American Gastroenterological Association.)