Record Display for the EPA National Library Catalog


Main Title Interactions of Heavy Metals with the Pulmonary Metabolism of (3H)Benzo(a)pyrene.
Author Williams, S. J. ; Karis, M. A. ; Menzel, D. B. ;
CORP Author Duke Univ. Medical Center, Durham, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-806337; EPA/600/J-84/386;
Stock Number PB87-145546
Additional Subjects Carcinogens ; Metabolism ; Exposure ; Laboratory animals ; Cigarette smoking ; Reprints ; Benzopyrene ; Heavy metals ; Health effects ; Inhalation
Library Call Number Additional Info Location Last
NTIS  PB87-145546 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 18p
To test the hypothesis that the inhalation of heavy metal aerosols from polluted air or cigarette smoke could alter the metabolism of polycyclic aromatic hydrocarbons, isolated, ventilated, and perfused rat lungs were used to study the effects of NiCl2, CdCl2, and CoCl2 on the pulmonary metabolism of (3)H benzo(a)pyrene (3)H BAP. Five minutes prior to removing the lungs from a rat, 100 microliter of isotonic sucrose containing 1 micromole of NiCl2, CdCl2, or CoCl2 was instilled intratracheally. The lungs were placed in the perfusion system and washed free of blood. After 10 minutes, 5 nmole of (3)H BAP dissolved in 100 microliter of dimethylsulfoxide was introduced in the arterial circulation. After 45 minutes the lungs were homogenized and the homogenates and perfusates were extracted with ethyl acetate: (2:1, v/v) for analysis by HPLC. Binding of (3)H BAP metabolites to lung tissue was also determined. The metabolism of (3)H BAP was found to be inducible by pretreatment of rats with beta-naphthoflavone (80 mg/kg) and to be inhibited by the inclusion of metyrapone (1.0 mM) or indomethacin (0.1 mM) in the perfusate. The extent of covalent binding to lung macromolecules was proportional to the extent of metabolism of the (3)H BAP. Since heavy metals and polycyclic aromatic hydrocarbon coexist in the same atmospheric aerosols, heavy metal exposure from polluted urban or occupational air or cigarette smoking may have profound effects upon the bioactivity of a common organic carcinogen.