Record Display for the EPA National Library Catalog


Main Title Thiodiglycolic Acid: A Major Metabolite of Bis(2-chloro-ethyl)ether.
Author Lingg, Robert D. ; Kaylor, William H. ; Pyle, Steven M. ; Tardiff., Robert G. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1978
Report Number EPA/600/J-79/010;
Stock Number PB-295 384
Additional Subjects Toxicology ; Chemical analysis ; Dosage ; Rats ; Experimental data ; Laboratory animals ; Chlorine organic compounds ; Reprints ; Metabolites ; Urinalysis ; Quantitative analysis ; Ether/bis(chloroethyl) ; Diglycolic acid/thio ; Glucosiduronic acid/chloroethyl
Library Call Number Additional Info Location Last
NTIS  PB-295 384 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Male rats were given a single oral dose (40mg/kg) of bis(2-chloroethyl)ether (BCEE). Less than 2% of the dose was recovered from the expired air as the unaltered parent compound during an 8-hr collection period. Urine samples representing 48 hr of collection were analyzed by gas chromatography-mass spectrometry. Methyl ester/trimethylsilyl ether derivatives of the isolated urinary acid fraction were prepared for gasphase analysis. Two metabolites were identified in this fraction: thiodiglycolic acid (TDGA) and 2-chloroethyl beta-D-glucosiduronic acid. Quantitative analysis for TDGA gave an average (seven rats) yield for 48 hr of 33 + or - 11.8 mg (+ or - SD) of TDGA/kg from a single dose of 40 mg/kg BCEE. The glucuronide of 2-chloroethanol was synthesized using rabbit liver microsomes in an incubation mixture. The matching mass spectrum of the glucuronide prepared in vitro to that identified in the urine of the rats verified 2-chloroethyl Beta-D-glucosiduronic acid as a metabolite of BCEE.