Record Display for the EPA National Library Catalog


Main Title Uptake of Inorganic Lead In vitro by Isolated Mitochondria and Tissue Slices of Rat Renal Cortex.
Author Kapoor, S. C. ; van Rossum, G. D. V. ; O'Neill, K. J. ; Mercorella, I. ;
CORP Author Temple Univ., Philadelphia, PA. School of Medicine. ;Rome Univ. (Italy). Ist. di Patologia.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA-R-806616; EPA/600/J-85/157;
Stock Number PB86-100575
Additional Subjects Lead(Metal) ; Rats ; Tissues ; In vitro analysis ; Mitochondria ; Reprints ; Heavy metals
Library Call Number Additional Info Location Last
NTIS  PB86-100575 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Slices of rat renal cortex were shown to take up Pb2+ during incubation in vitro; Pb2+ was also shown to enter mitochondria within the slices. The uptake of Pb2+ by isolated mitochondria was inhibited by N3-, La3+ and ruthenium red. A steady state of uptake was attained within 60 sec. The concentration dependence of uptake was complex; maximum uptake was attained at 25 micromols and inhibition ensued at higher concentrations. A substantial inhibitor-resistant component of Pb2+ uptake was noted, especially at medium Pb2+ concentrations greater than 25 micromoles, and these concentrations also inhibited respiration state 3. The effects on respiration were reduced if the mitochondria had been preincubated with ruthenium red.