||Morphological and Physiological Studies of Rat Kidney Cortex Slices Undergoing Isosmotic Swelling and Its Reversal: A Possible Mechanism for Ouabain-Resistant Control of Cell Volume.
Russo, M. A. ;
Ernst, S. A. ;
Kapoor, S. C. ;
van Rossum, G. D. V. ;
||Rome Univ. (Italy). Ist. di Patologia Generale. ;Michigan Univ., Ann Arbor. Medical School. ;Temple Univ., Philadelphia, PA. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Laboratory animals ;
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Slices of rat kidney cortex were induced to swell by preincubation at 1C in an isotonic Ringer's solution, and their capacity to reverse swelling, by net extrusion of cellular water, was studied during subsequent incubation at 25C. The recovery from swelling was prevented by the respiratory inhibitor, antimycin A. On the other hand, extrusion of water was little affected by ouabain. The extrusion of water continuing in the presence of ouabain (but not that in its absence) was significantly reduced when furosemide was added or when medium Cl- was replaced by NO-3 or I-. There was substantial variability in the morphological appearance of cells within the cortical slices.