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Main Title Genotoxicity of 2-Amino-6-N-Hydroxyadenine (AHA) to Mouse Lymphoma and CHO Cells.
Author Moore, M. M. ; Harrington-Brock, K. ; Parker, L. ; Doerr, C. L. ; Hozier, J. C. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Florida Inst. of Tech., Melbourne. Medical Genetics Lab.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/J-91/213;
Stock Number PB91-243048
Additional Subjects Mutagens ; Lymphoma ; Mutation ; Mice ; Chinese hamsters ; Thymidine kinase ; Chromosome aberrations ; Micronucleus tests ; Karyotyping ; Hypoxanthine phosphoribosyltransferase ; Ouabain ; Chromosome mapping ; Reprints ; Aminohydroxyadenines
Library Call Number Additional Info Location Last
NTIS  PB91-243048 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
2-Amino-6-N-hydroxyadenine (AHA) treated L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells were evaluated for mutations at the tk, hgprt, and Na(1+)/K(1+) ATPase loci, as well as for gross chromosome aberrations and induction of micronuclei. In addition, AHA was evaluated for its ability to induce HGPRT mutants in CHO cells. AHA was found to induce mutations at all evaluated loci and in both cell types. The TK mutants were primarily large colonies although a few small colonies were also induced, particularly at the higher concentrations. Preliminary cytogenetic analysis of AHA-treated mouse lymphoma cells indicated that some gross aberrations but not micronuclei were induced. The 20 small-colony TK mutants evaluated by banded karyotype indicate that only a small fraction (2 of 20) showed chromosome 11 abnormalities. From these studies, it appears that AHA may be one of a very few chemicals that is capable of inducing multi-locus point mutations, with only slight clastogenic activity. Particularly at the higher concentrations, some of the mutants may contain multi-locus point mutations that result in slow growth. (Copyright (c) 1991 Elsevier Science Publishers B.V.)