Record Display for the EPA National Library Catalog


Main Title Formation of Cigarette Smoke-Induced DNA Adducts in the Rat Lung and Nasal Mucosa.
Author Gupta, R. C. ; Sopori, M. L. ; Gairola., C. G. ;
CORP Author Baylor Coll. of Medicine, Houston, TX. Dept. of Pharmacology. ;Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM. ;Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/193;
Stock Number PB90-140799
Additional Subjects Deoxyribonucleic acids ; Smoking ; Lung ; Liver ; Rats ; Respiration ; Exposure ; Blood ; Reprints ; DNA adducts ; Nasal mucosa ; DNA damage ; Dose-response relationships ; Carboxyhemoglobin
Library Call Number Additional Info Location Last
NTIS  PB90-140799 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The formation of DNA adducts in the nasal, lung, and liver tissues of rats exposed daily to fresh smoke from a University of Kentucky reference cigarette (2R1) for up to 40 weeks was examined. The amount of smoke total particulate matter (TPM) inhaled and the blood carboxyhemoglobin (COHb) values averaged 5-5.5 mg smoke TPM/day/rat and 5.5%, respectively. The pulmonary AHH activity measured at the termination of each experiment showed an average increase of about two- to threefold in smoke-exposed groups. These observations suggested that animals effectively inhaled both gaseous and particulate phase constituents of cigarette smoke. DNAs from nasal, lung, and liver tissue were extracted and analyzed by an improved 32P-postlabeling procedure. The data demonstrates the DNA-damaging potential of long term fresh cigarette smoke exposure and suggest the ability of the tissue to partially recover from such damage following cessation of the exposure.