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Main Title Cognitive and Neuroanatomical Effects of Triethyltin in Developing Rats: Role of Age of Exposure. Research Report.
Author Freeman, J. H. ; Barone, S. ; Stanton, M. E. ;
CORP Author North Carolina Univ. at Chapel Hill. Dept. of Psychology. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Environmental Protection Agency, Research Triangle Park, NC. Neurotoxicology Div.
Publisher cFeb 94
Year Published 1994
Report Number EPA-68-D2-0056; EPA/600/J-94/321;
Stock Number PB94-197159
Additional Subjects Triethyltin ; Toxicology ; Brain ; Learning ; Exposure ; Growth ; Anatomy ; Glial fibrillary acid protein ; Immunohistochemistry ; Animal behavior ; Histology ; Rats ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-197159 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Long-Evans rat pumps were injected i.p. on postnatal day 5 (PND5) or 12 with 0, 3, or 5 mg/kg triethyltin sulfate (TET) and then tested on T-maze delayed alternation on PND21 or 28. Delayed alternation learning was impaired on PND21 and 28 in pups given 5 mg/kg TET. Pups given 5 mg/kg TET on PND5 were more impaired on delayed alternation than pups given 5 mg/kg TET on PND12. Pups given 3 mg/kg TET on PND5 or 12 were unimpaired at either age of testing. On the day following training, pups were sacrificed for histological assessment employing Nissl- or immunohistochemical staining for glial fibrillary acidic protein (GFAP), a putative marker of gliosis. Pups given 5 mg/kg TET on PND5 showed increases in GFAP immunoreactivity (IR) in subiculum, amygdala, hippocampus, piriform cortex, and entorhinal cortex with concomitant decreases in Nissl-stained cells in these regions. Pups given 5 mg/kg TET on PND12 showed increases in GFAP IR in piriform cortex amygdala and dorsal hippocampus with concomitant decreases in Nissl-stained cells in these regions. Exposure to 3 mg/kg TET on PND5 and PND12 produced a mild increase in GFAP IR in piriform cortex and amygdala but no discernible loss of Nissl-staining in these respective regions. TET-induced behavioral deficits appear related to damage of structural correlates of the human temporal lobe and not piriform cortical pathology. These results demonstrate that the day of exposure greatly influences the mangitude of the cognitive deficits and neuropathology associated with exposure to TET.