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Main Title DNA Adducts in Rat Lung, Liver and Peripheral Blood Lymphocytes Produces by i.p. Administration of Benzo(a)Pyrene Metabolites and Derivatives.
Author Ross, J. ; Nelson, G. ; Erexson, G. ; Kligerman, A. ; Earley, K. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/J-91/306;
Stock Number PB92-124627
Additional Subjects Deoxyribonucleic acids ; Lung ; Liver ; Lymphocytes ; Benzo(a)pyrene ; DNA damage ; Rats ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-124627 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
DNA adducts produced in vivo in rat lung, liver, and peripheral blood lymphocytes following the i.p. administration of several synthetic benzo(a)pyrene (B(a)P) metabolites and ring-substituted derivatives have been analyzed by the nuclease P1 version of the 32P-postlabeling assay. These include 1-,2-,3-,4-,5-,6-,7-,8-,9-,10-,11-, and 12-hydroxyB(a)P, (+ or -) -B(a)P-trans-4,5-dihydrodiol, (+ or -) -B(a)P-trans-7,8-dihydrodiol, (+ or -) -B(a)P-trans-9,10-dihydrodoil, and B(a)P-7,8-dione. Among the monohydroxy derivatives, only 2-,9-, and 12-hydroxyB(a)P produced detectable adducts. The only disubstituted derivative studied which produced adducts was the trans-7,8-dihydrodiol. The resulting DNA adducts were compared to those produced in each tissue by administration of B(a)P. 9-hydroxyB(a)P and B(a)P-trans-7,8-dihydrodiol each lead to the formation of major B(a)P adducts seen in lung and liver, respectively. None of the adducts derived from either 2-hydroxyB(a)P or 12-hydroxyB(a)P were observed following administration of B(a)P alone.