| Main Title |
Mutagenicity and Clastogenicity of Acrylamide in L5178Y Mouse Lymphoma Cells. |
| Author |
Moore, M. M. ;
Amtower, A. ;
Doerr, C. ;
Brock, K. H. ;
Dearfield, K. L. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| Year Published |
1987 |
| Report Number |
EPA/600/J-87/193; |
| Stock Number |
PB88-166004 |
| Additional Subjects |
Toxicology ;
Reprints ;
Mutagenesis ;
Clastogenesis ;
Acrylamide
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-166004 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Acrylamide was tested without exogenous activation in L5178Y/TK+/- 3.7.2C cells for mutation at the thymidine kinase locus and for clastogenicity. Acrylamide gave a positive induced mutagenic response (approximately 70 mutants/10 to the 6th power survivors) when tested at 600-650 micrograms/ml. The highest dose tested (850 micrograms/ml) resulted in an induced mutant frequency of approximately 380 mutants/10 to the 6th power survivors (survival = 13%). Acrylamide induced almost exclusively small-colony mutants, indicating that it might be acting by a clastogenic mechanism. As predicted, acrylamide was clastogenic, inducing both chromatid and chromosome breaks and rearrangements. A clearly positive clastogenic response was observed at both the 750 micrograms/ml and 850 micrograms/ml doses, which showed 16 and 64 aberrations per 100 cells respectively (background = 3 aberrations/ 100 cells). These studies indicate that the L5178Y/TK+/- mouse lymphoma assay can detect some chromosomal mutagens (clastogens) that show little activity in other single gene mutation assays, the CHO/HPRT and Salmonella. |
