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Main Title Analysis of DNA Strand Breaks Induced in Rodent Liver In vivo, Hepatocytes in Primary Culture, and a Human Cell Line by Chlorinated Acetic Acids and Chlorinated Acetaldehydes.
Author Chang, L. W. ; Daniel, F. B. ; DeAngelo, A. B. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-93/108;
Stock Number PB93-180974
Additional Subjects DNA damage ; Liver ; Acetic acids ; Aldehydes ; Chlorohydrocarbons ; Rats ; Cultured cells ; Mice ; Mutagens ; Humans ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-180974 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
An alkaline unwinding assay was used to quantitate the induction of DNA strand breaks (DNA SB) in the livers of rats and mice treated in vivo, in rodent hepatocytes in primary culture, and in CCRF-CEM cells, a human lymphoblastic leukemia cell line, following treatment with tri- (TCA), di- (DCA), and mono- (MCA) chloroacetic acid and their corresponding aldehydes, tri- (chloral hydrate, CH), di- (DCAA) and mono- (CAA) chloroacetaldehyde. The continuous exposure of mice to 5 g/L DCA in the drinking water for 7 and 14 days did not induce appreciable hepatic DNA SB (< 10% at 14 days), although peroxisome proliferation, as evidenced by an increased cyanide-insensitive palmitoyl CoA oxidase (PCO) activity, was stimulated to 490% (7 days) and 652% (14 days) of control. Under this protocol, DENA (0.1 g/L) produced DNA damage after both 7 days (73% of control) and 14 days (57% of control).