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Main Title Quantitative Image Cytometry of Hepatocytes Expressing Gamma-Glutamyl Transpeptidase and Glutathione S-Transferase in Diethylnitrosamine-Initiated Rats Treated with Phenobarbitol and/or Phthalate Esters.
Author Carter, J. H. ; Richmond, R. E. ; Carter, H. W. ; Potter, C. L. ; Daniel, F. B. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. Genetic Toxicology Div. ;Wood Hudson Cancer Research Lab., Newport, KY. ;Northern Kentucky Univ., Highland Heights. Dept. of Biological Sciences.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/317;
Stock Number PB92-227586
Additional Subjects Liver ; Gamma-glutamyltransferase ; Glutathione transferases ; Diethylnitrosamine ; Phenobarbital ; Cells(Biology) ; Rats ; Immunohistochemistry ; Carcinogens ; Pathology ; Reprints ; Phthalate esters ; Image cytometry
Library Call Number Additional Info Location Last
NTIS  PB92-227586 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Image cytometry was used to quantify the volume of liver tissue expressing two widely accepted biochemical markers of neoplasia, gammaglutamyl transpeptidase (GGT) and the placental isozyme of glutathione s-transferase (GST-P). Rats were treated with the hepatocarcinogen, diethylnitrosamine (DENA) alone or combined with phenobarbital (BP). Other groups of rats received either diethylhexylphthalate (DEHP) or di-n-octylphthalate (DOP) in their diets for 26 weeks. GGT expression was detected diffusely throughout the liver parenchyma in several treatment groups so that any enhanced expression in AF and N was not apparent. GST-P was detected only in AF and N. GST-P may represent a second genetic alteration as GST-P(+) AF and N also expressed GGT but not the reverse. By using the stereologic Principle of Delesse (area%=volume%) and the known liver weight, the grams of tissue expressing either marker was quantified. The peroxisome proliferator, DEHP, inhibited expression of GGT or GST-P in livers of either DENA treated or DENA+PB treated rats. With GST-P, the reduction was correlated to a reduced number of AF and N.