Record Display for the EPA National Library Catalog


Main Title Genotoxicity of 2,4,5-Trichlorophenoxyacetic Acid Biodegradation Products in the 'Salmonella' Reversion and Lambda Prophage-Induction Bioassays.
Author George, S. E. ; Whitehouse, D. A. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/372;
Stock Number PB93-107175
Additional Subjects Mutagens ; Salmonella ; Bioassay ; Phage lambda ; Metabolic activation ; Biodeterioration ; Pseudomonas cepacia ; Tables(Data) ; Reprints ; Trichlorophenoxyacetic acids
Library Call Number Additional Info Location Last
NTIS  PB93-107175 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Pseudomonas cepacia strain AC1100 has been isolated and reported to utilize 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as sole carbon and energy source. Metabolites from the 2,4,5-T degradation pathway were tested for their mutagenicity in the Salmonella reversion bioassay and genotoxicity in the prophage-induction bioassay. The parental compound (2,4,5-T) and three reported metabolites (2,4,5-trichlorophenol, 2,5-dichlorohydroquinone, and 2-chlorosuccinate) were negative with and without metabolic activation in Salmonella typhimurium strains TA98, TA100, TA102, and TA104. Conversely, 2,4,5-T and 2,4,5-trichlorophenol were positive in the prophage-induction bioassay with added S9. 2,4,5-Trichlorophenol was approximately 100-fold more genotoxic than 2,4,5-T 2,5-Dichlorohydroquinone was a weak direct-acting genotoxicant in the assay. In order to determine if strain AC1100 eliminated genotoxicity, 2,4,5-T medium was inoculated and the genotoxicity and the amount of 2,4,5-T remaining were examined over the growth period. As cell numbers increased, the percentage of 2,4,5-T remaining in the medium decreased. The decrease coincided with a decrease in genotoxicity in the prophage-induction bioassay. No mutagenic response was observed in the Salmonella reversion assay.