Record Display for the EPA National Library Catalog


Main Title Assessing the Effect of Colony Counting Methods and Genetic Drift on Ames Bioassay Results.
Author Claxton, L. D. ; Toney, S. ; Perry, E. ; King, L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1984
Report Number EPA/600/J-84/083;
Stock Number PB84-244151
Additional Subjects Counting ; Bacteria ; Salmonella ; Bioassay ; Assessments ; Reprints ; Ames test ; Colony counting
Library Call Number Additional Info Location Last
NTIS  PB84-244151 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Accuracy of automatic colony counters and variation among subcultures of the Salmonella tester strains are two factors that cause variation within the Ames bioassay. This paper examines these potential sources of variation. In contrast to results seen by other researchers, this genetic drift study demonstrated that except for TA100 the subcultures of tester strains from four research laboratories are very stable in biochemical characteristics measured using Analytical Profile Index (API) strips. This study also documented the levels of accuracy for hand- and automatic-counting techniques, and provides a simple method for generating acetate calibration transparencies for use with most colony counters.