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Main Title Detection of Giardia muris and Giardia lamblia Cysts by Immunofluorescence in Animal Tissues and Fecal Samples Subjected to Cycles of Freezing and Thawing.
Author Erlandsen, S. L. ; Sherlock, L. A. ; Bemrick., W. J. ;
CORP Author Minnesota Univ., Minneapolis. Medical School. ;Minnesota Univ., St. Paul. Coll. of Veterinary Medicine.;Environmental Monitoring Systems Lab., Cincinnati, OH.
Publisher c1990
Year Published 1990
Report Number EPA-R-814622; EPA/600/J-90/095;
Stock Number PB90-245366
Additional Subjects Giardia ; Tissues(Biology) ; Feces ; Parasitology ; Freezing ; Cysts ; Melting ; Reprints ; Giardia muris ; Giardia lamblia ; Fluorescent antibody technic ; Scanning electron microscopy
Library Call Number Additional Info Location Last
NTIS  PB90-245366 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The effects of freezing and thawing on the detection of selected Giardia spp. cysts were investigated using immunofluorescence, bright field microscopy, and low voltage scanning electron microscopy (SEM). Giardia muris cysts were obtained from either animal carcasses, fecal pellets, or isolated cyst preparations, while Giardia lamblia cysts were isolated from fecal samples. These samples were stained using an immunofluorescence technique after 1 to 3 freezing (-16 C) and thawing (20 C) cycles. Cysts were detected successfully by immunofluorescence in all samples. However, in those samples subjected to freeze-thawing, the cyst walls often became distorted and then were not detectable by bright field microscopy. Low voltage SEM demonstrated that the filaments in the distorted cyst wall underwent rearrangements of interfilament spacing. Quantitation of cyst recovery after freezing and thawing demonstrated that a substantial loss occurred after 1 cycle of alternating temperature when low concentrations of cysts were used, but not with high concentrations of cysts. Cyst recovery, after 3 freezing and thawing cycles, was dramatically lowered irrespective of the initial cyst concentration. (Copyright (c) American Society of Parasitologists.)