Record Display for the EPA National Library Catalog


Main Title Interspecies Cytogenetic Comparisons: Studies with X-Radiation and Bleomycin Sulfate.
Author Kligerman, A. D. ; Bryant, M. F. ; Doerr, C. L. ; Halperin, E. C. ; Kwanyuen, P. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC. Dept. of Radiation Oncology.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1992
Year Published 1992
Report Number EPA-68-02-4456; EPA/600/J-92/301;
Stock Number PB92-227438
Additional Subjects Chromosome aberrations ; Species diversity ; Bleomycin ; X rays ; Comparison ; In vitro analysis ; Lymphocytes ; Dose-response relationships ; Humans ; Rats ; Mice ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-227438 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
A series of in vitro experiments were conducted to determine if there are innate differences in the sensitivity of peripheral blood lymphocytes (PBLs) from different mammalian species to clastogens. Mouse, rat, and human whole blood samples were exposed to either 0, 0.38, 0.75, 1.5, or 3.0 Gy x-radiation or 0, 5, 10, 20, 40, or 80 micrograms/ml bleomycin for 4 h. Bromodeoxyuridine-containing cultures were initiated and the PBLs stimulated to divide with phytohemagglutinin. All cultures were harvested following a 3-h colcemid treatment. Slides were made and differentially stained, and first-division metaphases were scored for chromosome aberrations. In the x-radiation studies human PBLs were significantly more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs as measured by either the total percent aberrant cells or the number of dicentrics. Results with bleomycin suggest that the mouse and human PBLs are equally sensitive to the clastogenic effects of bleomycin. These results indicate that there may be inherent differences in sensitivity among PBLs of mammalian species; however, more studies are needed to determine if the differences presented here hold for other agents.