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Grantee Research Project Results

Publications Details for Grant Number R827084

The Development of a DNA Based Specific Assay for Pfiesteria piscicida in Water and Sediments

RFA: Ecology and Oceanography of Harmful Algal Blooms (1998)

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Book (2)
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Dissertation/Thesis (4)
Journal Article (17)
Paper (1)
Presentation (54)
Proceedings (8)
Report (1)
Reference Type Reference Title Journal Author Citation Progress Report Year Document Sources
Book Manual on Harmful Marine Microalgae. None Hallegraeff GM, Anderson DM, Cembella AD Hallegraeff GM, Anderson DM, Cembella AD, eds. Manual on Harmful Marine Microalgae. Monographs on Oceanographic Methodology 11. Paris, France: UNESCO, 2003, 793 pp. R827084 (Final)
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Book Rapid Cycle Real-Time PCR: Methods and Application None Meuer SC, Wittwer C, Nakagawara K Meuer SC, Wittwer C, Nakagawara K, eds. Rapid Cycle Real-Time PCR: Methods and Applications, Heidelberg, Germany: Springer-Verlag, 2001, 408 pp. R827084 (Final)
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Book Chapter Real-time PCR monitoring of estuarine water samples for Pfiesteria piscicida, a dinoflagellate associated with fish kills and human illness. None Bowers HA, Tengs T, Herrmann R, Oldach DW Bowers HA, Tengs T, Herrmann R, Oldach DW. Real-time PCR monitoring of estuarine water samples for Pfiesteria piscicida, a dinoflagellate associated with fish kills and human illness. In: Meuer S, Wittwer C, Nakagawara K, eds. Rapid Cycle Real-Time PCR: Methods and Applications. Heidelberg, Germany: Springer-Verlag, 2001, pp. 391-398. R827084 (2000)
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Book Chapter Pfiesteria piscicida and human health. None Oldach DW, Grattan LM, Morris JG Oldach DW, Grattan LM, Morris JG. Pfiesteria piscicida and human health. In:Scheld WM, Craig WA, Hughes JM, eds. Emerging Infections 3. Washington, DC: ASM Press, 1999, Chapter 9, pp. 135-152. R827084 (Final)
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Book Chapter Detection of HAB species using lectin, antibody, and DNA probes None Scholin C, Vrieling E, Peperzak L, Rhodes L, Rublee P, Hallegraeff GM, Anderson DM, Cembella AD Scholin C, Vrieling E, Peperzak L, Rhodes L, Rublee P. Detection of HAB species using lectin, antibody, and DNA probes. In: In: Hallegraeff GM, Anderson DM, Cembella AD (Eds.). Manual on Harmful Marine Microalgae. Intergovernmental Oceanographic Commission of UNESCO, Chapter 5, 2nd edition. R827084 (2001)
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Dissertation/Thesis Utilization of the polymerase chain reaction and fluorescent in situ hybridization to assess fine scale and global distribution patterns of Pfiesteria species None Allen CI Allen CI. Utilization of the polymerase chain reaction and fluorescent in situ hybridization to assess fine scale and global distribution patterns of Pfiesteria species. M.S. Thesis, Biology Department, University of North Carolina-Greensboro, Greensboro, NC, 2000, 101 pp. R827084 (2000)
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Dissertation/Thesis Development of a real-time PCR assay for detection and quantification of Pfiesteria piscicida organisms in environmental estuarine water samples None Bowers H Bowers H. Development of a real-time PCR assay for detection and quantification of Pfiesteria piscicida organisms in environmental estuarine water samples. M.S. Thesis, Environmental Science Program, The Johns Hopkins University, Baltimore, MD, 2000, 73 pp. R827084 (2000)
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Dissertation/Thesis Spatial and temporal distribution of Pfiesteria piscicida in coastal North Carolina waters. None Harris J Harris J. Spatial and temporal distribution of Pfiesteria piscicida in coastal North Carolina waters. M.S. Thesis. Biology Department, University of North Carolina-Greensboro, Greensboro, NC, 2002. R827084 (Final)
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Dissertation/Thesis PCR and FISH assays for the detection of Pfiesteria piscicida None Kempton JW Kempton JW. PCR and FISH assays for the detection of Pfiesteria piscicida. M.S. Thesis, Biology Department, University of North Carolina-Greensboro, Greensboro, NC, 1999, 83 pp. R827084 (2000)
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Journal Article Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. APPLIED AND ENVIRONMENTAL MICROBIOLOGY Bowers HA, Tengs T, Glasgow Jr HB, Burkholder JM, Rublee PA, Oldach DW Bowers HA, Tengs T, Glasgow Jr HB, Burkholder JM, Rublee PA, Oldach DW. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Applied and Environmental Microbiology 2000;66(11):4641-4648. R827084 (2000)
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Journal Article Neurobehavioral complaints of symptomatic persons exposed to Pfiesteria piscicida or morphologically related organisms MARYLAND MEDICAL JOURNAL (BALTIMORE, MD.: 1985) Grattan LM, Oldach D, Tracy JK, Greenberg DR Grattan LM, Oldach D, Tracy JK, Greenberg DR. Neurobehavioral complaints of symptomatic persons exposed to Pfiesteria piscicida or morphologically related organisms. Maryland Medical Journal 1998;47(3):127-129. R827084 (Final)
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Journal Article Human health risks of exposure to Pfiesteria piscicida. BIOSCIENCE Grattan LM, Oldach D, Morris JG Grattan LM, Oldach D, Morris JG. Human health risks of exposure to Pfiesteria piscicida. BioScience 2001;51(10):853-858. R827084 (Final)
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Journal Article Gastrointestinal and respiratory tract symptoms following brief environmental exposure to aerosols during a Pfiesteria-related fish kill. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A Haselow DT, Brown E, Tracy JK, Magnien R, Grattan LM, Morris Jr JG, Oldach DW Haselow DT, Brown E, Tracy JK, Magnien R, Grattan LM, Morris Jr JG, Oldach DW. Gastrointestinal and respiratory tract symptoms following brief environmental exposure to aerosols during a Pfiesteria-related fish kill. Journal of Toxicology and Environmental Health, Part A 2001;63(8):553-564. R827084 (2001)
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Journal Article Discovery of the toxic dinoflagellate Pfiesteria in northern European waters None Jakobsen KS, Tengs T, Vatne A, Bowers HA, Oldach DW, Burkholder JM, Glasgow Jr HB, Rublee PA, Klaveness D Jakobsen KS, Tengs T, Vatne A, Bowers HA, Oldach DW, Burkholder JM, Glasgow Jr HB, Rublee PA, Klaveness D. Discovery of the toxic dinoflagellate Pfiesteria in northern European waters. Proceedings of the Royal Society of London Series B: Biological Sciences 2002;269(1487):211-214. R827084 (2001)
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Journal Article Low abundance of the dinoflagellates, Pfiesteria piscicida, P. shumwayae, and Cryptoperidiniopsis spp., in South Carolina tidal creeks and open estuaries. ESTUARIES Lewitus AJ, Hayes KC, Willis BM, Burkholder JM, Glasgow HB, Holland AF, Maier PP, Rublee PA, Magnien R Lewitus AJ, Hayes KC, Willis BM, Burkholder JM, Glasgow HB, Holland AF, Maier PP, Rublee PA, Magnien R. Low abundance of the dinoflagellates, Pfiesteria piscicida, P. shumwayae, and Cryptoperidiniopsis spp., in South Carolina tidal creeks and open estuaries. Estuaries 2002;25(4):586-597. R826944 (2000)
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Journal Article Cohort studies of health effects among people exposed to estuarine waters: North Carolina, Virginia, and Maryland. ENVIRONMENTAL HEALTH PERSPECTIVES Moe CL, Turf E, Oldach D, Bell P, Hutton S, Savitz D, Koltai D, Turf M, Ingsrisawang L, Hart R, Ball JD, Stutts M, McCarter R, Wilson L, Haselow D, Grattan L, Morris JG, Weber DJ Moe CL, Turf E, Oldach D, Bell P, Hutton S, Savitz D, Koltai D, Turf M, Ingsrisawang L, Hart R, Ball JD, Stutts M, McCarter R, Wilson L, Haselow D, Grattan L, Morris JG, Weber DJ. Cohort studies of health effects among people exposed to estuarine waters:North Carolina, Virginia, and Maryland. Environmental Health Perspectives 2001;109(Suppl 5):781-786. R827084 (2001)
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Journal Article Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES Oldach DW, Delwiche CF, Jakobsen KS, Tengs T, Brown EG, Kempton JW, Schaefer EF, Bowers HA, Glasgow Jr HB, Burkholder JM, Steidinger KA, Rublee PA Oldach DW, Delwiche CF, Jakobsen KS, Tengs T, Brown EG, Kempton JW, Schaefer EF, Bowers HA, Glasgow Jr HB, Burkholder JM, Steidinger KA, Rublee PA. Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools. Proceedings of the National Academy of Sciences of the United States of America 2000;97(8):4303-4308. R825551 (Final)
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Journal Article "Real-time" polymerase chain reaction GASTROENTEROLOGY Oldach D Oldach D. "Real-time" polymerase chain reaction. Gastroenterology 1999;116(3):763-764. R827084 (Final)
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Journal Article Regarding Pfiesteria. HUMAN ORGANIZATION Oldach D Oldach D. Regarding Pfiesteria. Human Organization 1999;58(4):459-460. R827084 (Final)
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Journal Article Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand. NEW ZEALAND JOURNAL OF MARINE AND FRESHWATER RESEARCH Rhodes LL, Burkholder JM, Glasgow HB, Rublee PA, Allen C, Adamson JE Rhodes LL, Burkholder JM, Glasgow HB, Rublee PA, Allen C, Adamson JE. Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand. New Zealand Journal of Marine and Freshwater Research 2002;36(3):621-630. R827084 (Final)
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Journal Article PCR and FISH detection extends the range of Pfiesteria piscicida in estuarine waters. VIRGINIA JOURNAL OF SCIENCE Rublee PA, Kempton J, Schaefer E, Burkholder JM, Glasgow Jr HB, Oldach D Rublee PA, Kempton J, Schaefer E, Burkholder JM, Glasgow Jr HB, Oldach D. PCR and FISH detection extends the range of Pfiesteria piscicida in estuarine waters. Virginia Journal of Science 1999;50(4):325-335. R825551 (Final)
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Journal Article Use of molecular probes to assess geographic distribution of Pfiesteria species. ENVIRONMENTAL HEALTH PERSPECTIVES Rublee PA, Kempton JW, Schaefer EF, Allen C, Harris J, Oldach DW, Bowers H, Tengs T, Burkholder JM, Glasgow Jr HB Rublee PA, Kempton JW, Schaefer EF, Allen C, Harris J, Oldach DW, Bowers H, Tengs T, Burkholder JM, Glasgow Jr HB. Use of molecular probes to assess geographic distribution of Pfiesteria species. Environmental Health Perspectives 2001;109(Suppl 5):765-767. R825551 (Final)
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Journal Article Pfiesteria: harmful algal blooms as indicators of human:ecosystem interactions. ENVIRONMENTAL RESEARCH Silbergeld EK, Grattan L, Oldach D, Morris JG Silbergeld EK, Grattan L, Oldach D, Morris JG. Pfiesteria: harmful algal blooms as indicators of human:ecosystem interactions. Environmental Research 2000;82(2):97-105. R827084 (Final)
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Journal Article Classification and identification of Pfiesteria and Pfiesteria-like species. ENVIRONMENTAL HEALTH PERSPECTIVES Steidinger K, Landsberg J, Richardson RW, Truby E, Blakesley B, Scott P, Tester P, Tengs T, Mason P, Morton S, Seaborn D, Litaker W, Reece K, Oldach D, Haas L, Vasta G Steidinger K, Landsberg J, Richardson RW, Truby E, Blakesley B, Scott P, Tester P, Tengs T, Mason P, Morton S, Seaborn D, Litaker W, Reece K, Oldach D, Haas L, Vasta G. Classification and identification of Pfiesteria and Pfiesteria-like species. Environmental Health Perspectives 2001;109(Suppl 5):661-665. R826791 (Final)
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Journal Article Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay. MOLECULAR ECOLOGY Tengs T, Bowers HA, Ziman AP, Stoecker DK, Oldach DW Tengs T, Bowers HA, Ziman AP, Stoecker DK, Oldach DW. Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay. Molecular Ecology 2001;10(2):515-523. R827084 (2000)
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Journal Article Identical ribosomal DNA sequence data from Pfiesteria piscicida (Dinophyceae) isolates with different toxicity phenotypes. ENVIRONMENTAL RESEARCH Tengs T, Bowers HA, Glasgow Jr HB, Burkholder JM, Oldach DW Tengs T, Bowers HA, Glasgow Jr HB, Burkholder JM, Oldach DW. Identical ribosomal DNA sequence data from Pfiesteria piscicida (Dinophyceae) isolates with different toxicity phenotypes. Environmental Research 2003;93(1):88-91. R827084 (Final)
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Paper Pfiesteria spp. and "Pfiesteria-like organisms" in South Carolina estuaries. None Lewitus A, Burkholder J, Cary C, Glasgow Jr H, Hayes K, Holland A, Law J, Rublee P Lewitus A, Burkholder J, Cary C, Glasgow Jr H, Hayes K, Holland A, Law J, Rublee P. Pfiesteria spp. and "Pfiesteria-like organisms" in South Carolina estuaries. R827084 (2000)
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Presentation The origin of the peridinin dinoflagellate plastid None Bachvaroff T, Tengs T, Oldach D, Jakobsen K, Delwiche C Bachvaroff T, Tengs T, Oldach D, Jakobsen K, Delwiche C. The origin of the peridinin dinoflagellate plastid. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
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Presentation Development of real-time PCR assays for the detection of harmful algal bloom species in algal culture, environmental water and sediment samples. None Bowers HA, Tengs T, Schaefer E, Jakobsen K, Rublee PA, Oldach DW Bowers HA, Tengs T, Schaefer E, Jakobsen K, Rublee PA, Oldach DW. Development of real-time PCR assays for the detection of harmful algal bloom species in algal culture, environmental water and sediment samples. Presented at the Molecular Probe Technology for the Detection of Harmful Algae Meeting, Martin Ryan Institute, Galway, Ireland, May 20-24, 2002. R827084 (Final)
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Presentation Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates None Bowers H, Tengs T, Glasgow Jr H, Burkholder J, Rublee P, Oldach D Bowers H, Tengs T, Glasgow Jr H, Burkholder J, Rublee P, Oldach D. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
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Presentation Temporal and spatial distribution of Pfiesteria piscicida in Chesapeake Bay waters, 1999-2000 None Bowers H, Tengs T, Ziman A, Tango P, Goshorn D, Magnien R, Karrh R, Oldach D Bowers H, Tengs T, Ziman A, Tango P, Goshorn D, Magnien R, Karrh R, Oldach D. Temporal and spatial distribution of Pfiesteria piscicida in Chesapeake Bay waters, 1999-2000. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
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Presentation Physical and biological factors associated with temporal and spatial distribution of Pfiesteria piscicida in the Chesapeake Bay None Bowers H, Tengs T, Burkholder J, Rublee P, Magnien R, Goshorn D, Oldach D Bowers H, Tengs T, Burkholder J, Rublee P, Magnien R, Goshorn D, Oldach D. Physical and biological factors associated with temporal and spatial distribution of Pfiesteria piscicida in the Chesapeake Bay. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
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Presentation Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates None Bowers H, Tengs T, Glasgow Jr H, Burkholder J, Rublee P, Oldach D Bowers H, Tengs T, Glasgow Jr H, Burkholder J, Rublee P, Oldach D. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Presented at the CDC National Conference on Pfiesteria: From Biology to Public Health, Stone Mountain, GA, October 18-20, 2000. R827084 (2000)
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Presentation Temporal and spatial distribution of Pfiesteria piscicida in Chesapeake Bay waters, 1999-2000 None Bowers H, Tengs T, Ziman A, Tango P, Goshorn D, Magnien R, Karrh R, Oldach D Bowers H, Tengs T, Ziman A, Tango P, Goshorn D, Magnien R, Karrh R, Oldach D. Temporal and spatial distribution of Pfiesteria piscicida in Chesapeake Bay waters, 1999-2000. Presented at the CDC National Conference on Pfiesteria: From Biology to Public Health, Stone Mountain, GA, October 18-20, 2000. R827084 (2000)
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Presentation Extraction of dinoflagellate DNA from sediment cores and analysis of community structure based on 18S sequences None Bowers H, Schaefer E, Rublee P, Tengs T, Arnold A, Brush G, Oldach D Bowers H, Schaefer E, Rublee P, Tengs T, Arnold A, Brush G, Oldach D. Extraction of dinoflagellate DNA from sediment cores and analysis of community structure based on 18S sequences. Presented at the Coastal Monitoring Through Partnerships, Environmental Monitoring and Assessment Program Symposium, Pensacola Beach, FL, April 2001. R827084 (2001)
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Presentation Extraction of dinoflagellate DNA from sediment cores and analysis of community structure based on 18S sequences. None Bowers H, Schaefer E, Rublee P, Tengs T, Arnold A, Brush G, Oldach D Bowers H, Schaefer E, Rublee P, Tengs T, Arnold A, Brush G, Oldach D. Extraction of dinoflagellate DNA from sediment cores and analysis of community structure based on 18S sequences. Mycotoxins and Phycotoxins. Presented at the Gordon Research Conferences, Williams College, Williamstown, MA, June 2001. R827084 (2001)
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Presentation Comparison of PCR and standardized fish bioassays for detection of toxic Pfiesteria complex species in estuarine sediments. None Deamer NJ, Brejwo K, Parrow M, Rublee PA, Glasgow Jr HB, Burkholder JM Deamer NJ, Brejwo K, Parrow M, Rublee PA, Glasgow Jr HB, Burkholder JM. Comparison of PCR and standardized fish bioassays for detection of toxic Pfiesteria complex species in estuarine sediments. Presented at the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Presentation Global dispersal of microorganisms and pathogens: biofilms inside ballast-water tanks. None Dobbs FC, Johnson WP, Meyer AE, Rublee PA Dobbs FC, Johnson WP, Meyer AE, Rublee PA. Global dispersal of microorganisms and pathogens:biofilms inside ballast-water tanks. Presented at the 11th International Congress on Marine Corrosion and Biofouling, University of San Diego, San Diego, CA, July 21-26, 2002. R827084 (Final)
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Presentation Pfiesteria species identified in ballast water and residuals: a possible vector for introductions to coastal areas. None Doblin MA, Drake LA, Coyne KJ, Rublee PA, Dobbs FC Doblin MA, Drake LA, Coyne KJ, Rublee PA, Dobbs FC. Pfiesteria species identified in ballast water and residuals:a possible vector for introductions to coastal areas. Presented at the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Presentation Associations between the presence of ulcerated menhaden and Pfiesteria in Maryland None Goshorn D, Magnien R, Tango P, Michael B, Oldach D, Bowers H Goshorn D, Magnien R, Tango P, Michael B, Oldach D, Bowers H. Associations between the presence of ulcerated menhaden and Pfiesteria in Maryland. Presented at the Estuarine Research Conference, St. Pete Beach, FL, November 4-8, 2001. R827084 (2001)
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Presentation Neurobehavioral recovery from toxic Pfiesteria exposure None Grattan L, Tracy J, Greenberg D, Fishburn C, Oldach D, Morris J Grattan L, Tracy J, Greenberg D, Fishburn C, Oldach D, Morris J. Neurobehavioral recovery from toxic Pfiesteria exposure. Presented at the Pfiesteria and the Environment: Convergence of Science and Policy Meeting, Georgetown, MD, December 10-11, 1998. R827084 (2000)
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Presentation Human health risks of exposure to Pfiesteria piscicida None Grattan L, Oldach D, Selnes O, Bever C, Civelek C, Wong D, Bagley T, Charache P, Morris J Grattan L, Oldach D, Selnes O, Bever C, Civelek C, Wong D, Bagley T, Charache P, Morris J. Human health risks of exposure to Pfiesteria piscicida. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
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Presentation Flu-like illness following environmental exposure to aerosols during a Pfiesteria-related fish kill None Haselow D, Brown E, Tracy J, Magnien R, Grattan L, Morris J, Oldach D Haselow D, Brown E, Tracy J, Magnien R, Grattan L, Morris J, Oldach D. Flu-like illness following environmental exposure to aerosols during a Pfiesteria-related fish kill. Presented at the CDC National Conference on Pfiesteria: From Biology to Public Health, Stone Mountain, GA, October 18-20, 2000. R827084 (2000)
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Presentation A descriptive account of potentially harmful algae in inland bays and Nanticoke River, Delaware. None Humphries E, Lacouture R, Gronefeld J, Marshall H Humphries E, Lacouture R, Gronefeld J, Marshall H. A descriptive account of potentially harmful algae in inland bays and Nanticoke River, Delaware. Delaware Department of Natural Resources and Environmental Control, Division of Water Resources, Environmental Laboratory Section, Dover, DE. Presented at the Symposium on Harmful Marine Algae in the United States, Woods Hole, MA, December 4-9, 2000. R827084 (Final)
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Presentation Pfiesteria piscicida and Pfiesteria shumwayae: associations with environmental parameters. None Humphries E Humphries E. Pfiesteria piscicida and Pfiesteria shumwayae:associations with environmental parameters. Pfiesteria Surveillance Monitoring Program 1999 Through 2001. Delaware Department of Natural Resources and Environmental Control. Presented at the Division of Water Resources, Environmental Laboratory Section, Dover, DE, December 9, 2002. R827084 (Final)
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Presentation Occurrence of potentially harmful micro-algae in Indian River/Bay and Rehoboth Bay, Delaware. None Humphries E Humphries E. Occurrence of potentially harmful micro-algae in Indian River/Bay and Rehoboth Bay, Delaware, 1999-2002. Delaware Department of Natural Resources and Environmental Control. Presented at the Division of Water Resources, Environmental Laboratory Section, Dover, DE, July 31, 2002. R827084 (Final)
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Presentation Phytoplankton characterization for Indian River/Bay and Rehoboth Bay, Delaware. None Humphries E Humphries E. Phytoplankton characterization for Indian River/Bay and Rehoboth Bay, Delaware, May through October 1999. Delaware Department of Natural Resources and Environmental Control. Presented at the Division of Water Resources, Environmental Laboratory Section, Dover, DE, May 31, 2002. R827084 (Final)
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Presentation HAB distribution and associations with environmental parameters: Delaware estuarine coastal bays. None Humphries E, Glasgow Jr HB, Marshall H, Lewitus AJ, Bennett-Wilde S Humphries E, Glasgow Jr HB, Marshall H, Lewitus AJ, Bennett-Wilde S. HAB distribution and associations with environmental parameters: Delaware estuarine coastal bays, US 1998-2002. Presented at the 10th International Harmful Algal Blooms Meeting, St. Petersburg, FL, 2002. R827084 (Final)
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Presentation State of Delaware HAB surveillance monitoring program. HAB associations with environmental parameters. None Humphries E Humphries E. State of Delaware HAB surveillance monitoring program. HAB associations with environmental parameters (1999-2001 composite data set). Presented at the Environmental Protection Agency Sponsored Workshop on Harmful Algal Blooms and Water Quality, Cambridge, MD, 2003. R827084 (Final)
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Presentation Fish lesions in the Chesapeake Bay: Pfiesteria-like dinoflagellates and other etiologies None Kane A, Oldach D, Reimschuessel R Kane A, Oldach D, Reimschuessel R. Fish lesions in the Chesapeake Bay:Pfiesteria-like dinoflagellates and other etiologies. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
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Presentation Fluorescent gene probes to Pfiesteria piscicida: results from the 1998 active season None Kempton J, Schaefer E, Burkholder J, Glasgow Jr H, Oldach D, Rublee P Kempton J, Schaefer E, Burkholder J, Glasgow Jr H, Oldach D, Rublee P. Fluorescent gene probes to Pfiesteria piscicida:results from the 1998 active season. Presented at the Pfiesteria and the Environment: Convergence of Science and Policy Meeting, Georgetown, MD, December 10-11, 1998. R827084 (2000)
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Presentation Pfiesteria, Pfiesteria-like species, and fish health in Florida: an update None Landsberg J, Steidinger K, Cook S, Singh E, Sosa E, Forstchen A, Wood R, Rublee P, Scott P, Wolny J, Bendis B Landsberg J, Steidinger K, Cook S, Singh E, Sosa E, Forstchen A, Wood R, Rublee P, Scott P, Wolny J, Bendis B. Pfiesteria, Pfiesteria-like species, and fish health in Florida: an update. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Pfiesteria, Pfiesteria-like species, and fish health in Florida None Landsberg J, Steidinger K, Cook S, Singh E, Sosa E, Forstchen A, Wood R, Rublee P, Scott P, Wolny J, Winner B, Whittington J, Perry N Landsberg J, Steidinger K, Cook S, Singh E, Sosa E, Forstchen A, Wood R, Rublee P, Scott P, Wolny J, Winner B, Whittington J, Perry N. Pfiesteria, Pfiesteria-like species, and fish health in Florida. Presented at the CDC National Conference on Pfiesteria: From Biology to Public Health, Stone Mountain, GA, October 18-20, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Pfiesteria shumwayae identified in Australian waters. None Lapworth C, LeRoi JM, Burkholder J, Rublee P Lapworth C, LeRoi J-M, Burkholder J, Rublee P. Pfiesteria shumwayae identified in Australian waters. Presented at the 16th Annual Conference for the Australian Society for Phycology and Aquatic Botany, Heron Island, Queensland, Australia, August 16-September 4, 2001. R827084 (2001)
not available
Presentation Pfiesteria spp. and "Pfiesteria-like organisms" in South Carolina estuaries None Lewitus A, Burkholder J, Cary C, Glasgow Jr H, Hayes K, Holland A, Law J, Rublee P Lewitus A, Burkholder J, Cary C, Glasgow Jr. H, Hayes K, Holland A, Law J, Rublee P. Pfiesteria spp. and "Pfiesteria-like organisms" in South Carolina estuaries. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2001)
R827084 (Final)
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Presentation Approaches to the investigation and interpretation of possible Pfiesteria-related events in Maryland None Magnien R, Goshorn D, Oldach D, Bowers H, Tengs T Magnien R, Goshorn D, Oldach D, Bowers H, Tengs T. Approaches to the investigation and interpretation of possible Pfiesteria-related events in Maryland. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Associations between Pfiesteria, fish health and environmental conditions in Chesapeake Bay None Magnien R, Goshorn D, Michael B, Burkholder J, Oldach D, Steidinger K, Driscoll C Magnien R, Goshorn D, Michael B, Burkholder J, Oldach D, Steidinger K, Driscoll C. Associations between Pfiesteria, fish health and environmental conditions in Chesapeake Bay. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Associations between Pfiesteria and environmental variables in Chesapeake Bay None Magnien R, Michael B, Tango P, Goshorn D, Karrh R, Trice M, Oldach D, Bowers H Magnien R, Michael B, Tango P, Goshorn D, Karrh R, Trice M, Oldach D, Bowers H. Associations between Pfiesteria and environmental variables in Chesapeake Bay. Presented at the Estuarine Research Conference, St. Pete Beach, FL, November 4-8, 2001. R827084 (2001)
R827084 (Final)
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Presentation Eutrophication and the distribution of Pfiesteria in urbanized tidal creeks. None Mallin MA, McIver MR, Ensign SH, Burkholder JM, Rublee PA Mallin MA, McIver MR, Ensign SH, Burkholder JM, Rublee PA. Eutrophication and the distribution of Pfiesteria in urbanized tidal creeks. Presented at the 10th International Harmful Algal Bloom Symposium, St. Petersburg, FL, October 2002. R827084 (Final)
not available
Presentation Molecular detection methods for identification of Pfiesteria piscicida and other estuarine toxin-producing dinoflagellates in field samples and algal cultures None Oldach D, Rublee P, Delwiche C, Glasgow Jr H, Jakobsen J, Tengs T, Bowers H, Brown E, Kempton J, Burkholder J Oldach D, Rublee P, Delwiche C, Glasgow Jr H, Jakobsen J, Tengs T, Bowers H, Brown E, Kempton J, Burkholder J. Molecular detection methods for identification of Pfiesteria piscicida and other estuarine toxin-producing dinoflagellates in field samples and algal cultures. Presented at the Gordon Research Conference on Mycotoxins and Phycotoxins, Plymouth, NH, June 20-25, 1999. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Development of molecular detection methods for Pfiesteria piscicida and other estuarine toxin-producing dinoflagellates guided by Heteroduplex Mobility Assay assisted sequence discovery None Oldach D, Rublee P, Delwiche C, Glasgow H, Jakobsen K, Tengs T, Bowers H, Brown E, Kempton J, Burkholder J Oldach D, Rublee P, Delwiche C, Glasgow H, Jakobsen K, Tengs T, Bowers H, Brown E, Kempton J, Burkholder J. Development of molecular detection methods for Pfiesteria piscicida and other estuarine toxin-producing dinoflagellates guided by Heteroduplex Mobility Assay assisted sequence discovery. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Molecular detection methods for Pfiesteria piscicida. None Oldach D Oldach D. Molecular detection methods for Pfiesteria piscicida. Presented at the State of Maryland Governor's Pfiesteria Technical Advisory Committee Meeting, Annapolis, MD, March 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Global distribution of toxic Pfiesteria complex species. None Rublee PA, Allen C, Schaefer E, Rhodes L, Adamson J, Lapworth C, Burkholder J, Glasgow H Jr Rublee PA, Allen C, Schaefer E, Rhodes L, Adamson J, Lapworth C, Burkholder J, Glasgow H Jr. Global distribution of toxic Pfiesteria complex species. Presented at the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Presentation Pfiesteria distribution and anthropogenic nutrient enrichment across large scales None Rublee PA, Harris J, Schaefer E, Burkholder JM, Glasgow HB, Oldach D, Magnien R, Goshorn D, Humphries E, Nuzzi R, Rhodes L, Simons JD, Villareal T Rublee PA, Harris J, Schaefer E, Burkholder JM, Glasgow HB, Oldach D, Magnien R, Goshorn D, Humphries E, Nuzzi R, Rhodes L, Simons JD, Villareal T. Pfiesteria distribution and anthropogenic nutrient enrichment across large scales. Presented at the Estuarine Research Federation Biennial Meeting, St. Petersburg, FL, November 4-8, 2001. R827084 (2001)
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Presentation Pfiesteria distribution and anthropogenic nutrient enrichment across large scales. None Rublee PA, Schaefer EF, Harris J, Burkholder JM, Glasgow HB Jr, Oldach D, Magnien R, Goshorn D, Humphries E, Nuzzi R, et al Rublee PA, Schaefer EF, Harris J, Burkholder JM, Glasgow HB Jr, Oldach D, Magnien R, Goshorn D, Humphries E, Nuzzi R, et al. Pfiesteria distribution and anthropogenic nutrient enrichment across large scales. Presented at the North Carolina Academy of Sciences Annual Meeting, University of North Carolina, Chapel Hill, NC, March 23-24, 2002. R827084 (Final)
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Presentation Pfiesteria species: what have we learned in twelve years? None Rublee PA Rublee PA. Pfiesteria species: what have we learned in twelve years? Cawthron Institute of Marine and Freshwater Research, Nelson, New Zealand, October 16, 2001. R827084 (2001)
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Presentation Pfiesteria species: what have we learned in twelve years? None Rublee PA Rublee PA. Pfiesteria species: what have we learned in twelve years? Presented at the Commonwealth Scientific and Industrial Research Organization, Hobart Tasmani, Australia, October 8, 2001. R827084 (2001)
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Presentation Distribution of Pfiesteria piscicida and two associated dinoflagellates along the U.S. East Coast during the active season in 1998 and 1999 None Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D. Distribution of Pfiesteria piscicida and two associated dinoflagellates along the U.S. East Coast during the active season in 1998 and 1999. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Development of ribosomal gene probes for detection of Pfiesteria piscicida None Rublee P Rublee P. Development of ribosomal gene probes for detection of Pfiesteria piscicida. Presented at the Gordon Research Conference on Mycotoxins and Phycotoxins, Plymouth, NH, June 20-25, 1999. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Distribution of Pfiesteria species: comparison of results from water and sediment samples across multiple scales, 1889-2000 None Rublee P, Schaefer E, Allen C, Harris J, Bowers H, Tengs T, Oldach D Rublee P, Schaefer E, Allen C, Harris J, Bowers H, Tengs T, Oldach D. Distribution of Pfiesteria species: comparison of results from water and sediment samples across multiple scales, 1889-2000. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Distribution of Pfiesteria spp. along the U.S. east coast during 1998 and 1999 None Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D. Distribution of Pfiesteria spp. along the U.S. east coast during 1998 and 1999. Presented at the 5th Symposium of the National Health and Environmental Effects Research Laboratory, Research Triangle Park, NC, June 6-8, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Distribution of Pfiesteria spp. and related dinoflagellates along the U.S. east coast during 1999 None Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D Rublee P, Kempton J, Schaefer E, Allen C, Burkholder J, Glasgow Jr H, Oldach D. Distribution of Pfiesteria spp. and related dinoflagellates along the U.S. east coast during 1999. Presented at the Atlantic Estuarine Research Society/Southeast Estuarine Research Society Meeting, Wilmington, NC, March 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Distribution of Pfiesteria spp. and related dinoflagellates along the U.S. east coast during 1999. None Rublee P Rublee P. Distribution of Pfiesteria spp. and related dinoflagellates along the U.S. east coast during 1999. Presented at the State of Maryland Governor's Pfiesteria Technical Advisory Committee Meeting, Annapolis, MD, March 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Prevention, Control and Mitigation of Harmful Algal Blooms: a research plan None Rublee P Rublee P. Prevention, Control and Mitigation of Harmful Algal Blooms: a research plan. Presented at the Sea Grant Workshop on Prevention, Control, and Mitigation of Harmful Algal Blooms, Washington, DC, May 7-8, 2001. R827084 (2001)
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Presentation Toxic Pfiesteria strains found in shellfish from estuaries known for toxic Pfiesteria activity: implications for seafood safety. None Shumway SE, Springer J, Rublee PA, Glasgow HB Jr, Burkholder JM Shumway SE, Springer J, Rublee PA, Glasgow HB Jr, Burkholder JM. Toxic Pfiesteria strains found in shellfish from estuaries known for toxic Pfiesteria activity: implications for seafood safety. Presented at the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Presentation Pfiesteria piscicida: Identifying toxins associated with a novel human neurotoxic syndrome None Silbergeld E, Eldefrawi A, Sass J, El Nabawi A, Choich J, Sathyanarayana S, Brewer V, Conrad S, Eilers P, Kane A, Oldach D, Hall S, Morris J Silbergeld E, Eldefrawi A, Sass J, El Nabawi A, Choich J, Sathyanarayana S, Brewer V, Conrad S, Eilers P, Kane A, Oldach D, Hall S, Morris J. Pfiesteria piscicida: identifying toxins associated with a novel human neurotoxic syndrome. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation High evolutionary rates in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay None Tengs T, Bowers H, Ziman A, Stoecker D, Oldach D Tengs T, Bowers H, Ziman A, Stoecker D, Oldach D. High evolutionary rates in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay. Presented at the Symposium on Harmful Marine Algae in the U.S., Woods Hole, MA, December 4-9, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Phylogenetic analyses indicate that the 19`hexanoyloxy-fucoxanthin-containing dinoflagellates have tertiary plastids of haptophyte origin None Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K. Phylogenetic analyses indicate that the 19`hexanoyloxy-fucoxanthin-containing dinoflagellates have tertiary plastids of haptophyte origin. Presented at the Gordon Research Conference on Mycotoxins and Phycotoxins, Plymouth, NH, June 20-25, 1999. R827084 (2000)
R827084 (2001)
R827084 (Final)
not available
Presentation Phylogenetic analyses indicate that the 19`hexanoyloxy-fucoxanthin-containing dinoflagellates have tertiary plastids of haptophyte origin None Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K. Phylogenetic analyses indicate that the 19`hexanoyloxy-fucoxanthin-containing dinoflagellates have tertiary plastids of haptophyte origin. Presented at the 9th International Conference on Harmful Algal Blooms, Tasmania, Australia, February 7-11, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Phylogeny of the dinoflagellates and their endosymbionts. None Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K Tengs T, Dahlberg O, Shalchian-Tabrizi K, Klaveness D, Oldach D, Delwiche C, Jakobsen K. Phylogeny of the dinoflagellates and their endosymbionts. Presented at the 13th Meeting of the International Society for Evolutionary Protistology, Czech Republic, July 31-August 4, 2000. R827084 (2000)
R827084 (2001)
R827084 (Final)
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Presentation Pfiesteria distribution along the Texas coast. None Villareal T, Simons JD, Rublee P Villareal T, Simons JD, Rublee P. Pfiesteria distribution along the Texas coast. Presented at the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Development of real-time PCR assays for the detection of Chattonella species in culture and environmental samples. None Bowers H, Tengs T, Goto S, Tomas C, Ono C, Yoshimatsu S, Oldach D Bowers H, Tengs T, Goto S, Tomas C, Ono C, Yoshimatsu S, Oldach D. Development of real-time PCR assays for the detection of Chattonella species in culture and environmental samples. In: Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Pfiesteria species identified in ballast water and residuals: a possible vector for introductions to coastal areas. None Doblin MA, Drake LA, Coyne KJ, Rublee PA, Dobbs FC Doblin MA, Drake LA, Coyne KJ, Rublee PA, Dobbs FC. Pfiesteria species identified in ballast water and residuals:a possible vector for introductions to coastal areas. In: Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Chronic urea nitrogen loading: a correlate of Pfiesteria spp. in the Chesapeake and coastal bays of Maryland, USA. None Glibert PM, Alexander J, Trice TM, Michael B, Magnien RE, Lane L, Oldach D, Bowers H Glibert PM, Alexander J, Trice TM, Michael B, Magnien RE, Lane L, Oldach D, Bowers H. Chronic urea nitrogen loading: a correlate of Pfiesteria spp. in the Chesapeake and coastal bays of Maryland, USA. In: Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Low abundance of the dinoflagellates, Pfiesteria piscicida, P. shumwayae, and Cryptoperidinopsis spp. in South Carolina estuaries: relevance as reference sites to areas impacted by Pfiesteria toxic events None Lewitus AJ, Hayes KC, Willis BM, Burkholder JM, Holland AF, Rublee PA, Magnien R Lewitus AJ, Hayes KC, Willis BM, Burkholder JM, Holland AF, Rublee PA, Magnien R. Low abundance of the dinoflagellates, Pfiesteria piscicida, P. shumwayae, and Cryptoperidinopsis spp. in South Carolina estuaries: relevance as reference sites to areas impacted by Pfiesteria toxic events. In: Hallegraeff G.M., Blackburn S., Bolch C., Lewis R. (eds.) Proceedings of the 9th International Conference on Harmful Algal Blooms. Paris: Intergovernmental Oceanographic Commission of UNESCO. R827084 (2001)
R827084 (Final)
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Proceedings Eutrophication and the distribution of Pfiesteria in urbanized tidal creeks. None Mallin MA, McIver MR, Ensign SH, Burkholder JM, Rublee PA Mallin MA, McIver MR, Ensign SH, Burkholder JM, Rublee PA. Eutrophication and the distribution of Pfiesteria in urbanized tidal creeks. In: Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Distribution of Pfiesteria sp. and an associated dinoflagellate along the U.S. East Coast during the active season in 1998 and 1999. None Rublee PA, Kempton JW, Schaefer EF, Allen C, Burkholder JM, Glasgow HB, Oldach DW Rublee PA, Kempton JW, Schaefer EF, Allen C, Burkholder JM, Glasgow HB, Oldach DW. Distribution of Pfiesteria sp. and an associated dinoflagellate along the U.S. East Coast during the active season in 1998 and 1999. In: Hallegraeff GM, Blackburn S, Bolch C, Lewis R, eds. Proceedings of the 9th International Conference on Harmful Algal Blooms. Paris:Intergovernmental Oceanographic Commission of UNESCO. R827084 (2001)
R827084 (Final)
not available
Proceedings Global distribution of pfiesteria-complex species. None Rublee PA, Allen C, Schaefer EF, Rhodes L, Adamson J, Burkholder JM, Glasgow H Rublee PA, Allen C, Schaefer EF, Rhodes L, Adamson J, Burkholder JM, Glasgow H. Global distribution of pfiesteria-complex species. In: Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Proceedings Pfiesteria distribution along the Texas coast. None Villareal T, Simons JD, Rublee PA Villareal T, Simons JD, Rublee PA. Pfiesteria distribution along the Texas coast. In:Proceedings of the 10th International Conference on Harmful Algal Blooms, St. Petersburg, FL, October 2002. R827084 (Final)
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Report Results from Pfiesteria-related water quality monitoring in Delaware's segment of the tidal Nanticoke River/Broad Creek system during 1998-99. None Tyler R Tyler R. Results from Pfiesteria-related water quality monitoring in Delaware's segment of the tidal Nanticoke River/Broad Creek system during 1998-99. March 2003 Draft Report. Delaware Department of Natural Resources and Environmental Control, Division of Water Resources, Environmental Laboratory Section, Dover, DE, 2003. R827084 (Final)
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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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