Grantee Research Project Results
2000 Progress Report: Use of a Transgenerational Model to Evaluate Threshold Effects of Endocrine Disrupting Chemicals in Fish
EPA Grant Number: R827098Title: Use of a Transgenerational Model to Evaluate Threshold Effects of Endocrine Disrupting Chemicals in Fish
Investigators: Foran, Christy M.
Current Investigators: Foran, Christy M. , Slattery, Marc
Institution: University of Mississippi
EPA Project Officer: Hahn, Intaek
Project Period: October 1, 1998 through September 30, 2001
Project Period Covered by this Report: October 1, 1999 through September 30, 2000
Project Amount: $406,024
RFA: Exploratory Research - Environmental Biology (1998) RFA Text | Recipients Lists
Research Category: Biology/Life Sciences , Environmental Justice , Human Health , Aquatic Ecosystems
Objective:
The primary objective of the proposed research is to link whole animal reproductive impairment to alterations in morphological development and/or endocrine function following exposure to model endocrine disrupting chemicals. These model chemicals have different mechanisms of action, but will be assessed with the same endpoints to determine if there are common predictors of developmental or reproductive toxicity, or if different mechanisms induce different suites of biochemical/endocrine changes thereby providing a "fingerprint" of the mechanisms.Progress Summary:
We currently have finished testing the second and starting the third of five model endocrine disruptors on reproduction in Japanese medaka. The five model chemicals were chosen based on their distinct mechanisms of action. To meet the objective set forth in the grant, we have exposed medaka to purported endocrine disruptors during two critical life stages: sexual differentiation and reproduction.Cadmium (Cd) has been tested as a model compound representing centrally-acting endocrine disruptors. In addition, ethinylestradiol (EE), a potent estrogen used in oral contraceptives has been tested. EE2 is distinguished from OP, another estrogen (E2) receptor agonist, because of its 1000 to 10000-fold greater affinity for the receptor, and because to its potential entry into the environment post-usage via sewage treatment plants (Desbrow, et al., 1996; Aherne and Briggs, 1989; for review see Arcand-Hoy, et al., 1998).
We finished our assessment of EE on reproductive function in medaka through exposure of larvae and adult pairs. The adults raised as a result of these exposures (F2 from adult exposure or mature animals from hatchling exposure) have been being assessed for changes in reproductive endpoints. These animals are exposed for 14 days at concentrations ranging from 0.2 ng/L to 2000 ng/L, including a concentration in the environmentally relevant range (5 ng/L). EE is not very acutely toxic to medaka; 2000 ng/L did not affect mortality. However, higher doses did arrest reproduction. We are currently evaluating the biochemical changes related to exposure.
Future Activities:
Our goal for Year 3 of this grant is to complete as much as possible the exposure for the three remaining chemicals. We have begun exposure to an environmental androgen, trenbolone. Trenbolone is an androgen fed to cattle to increase growth and reduce susceptibility to disease. We do not anticipate major changes in our use of equipment, techniques, or materials.References:
Aberne GW, Briggs R. The relevance of the presence of certain synthetic steroids in the aquatic environment. Journal of Pharmacy and Pharmacology 1989;41:735-736.
Arcand-Hoy LD, Nimrod AC, Benson WH. Endocrine modulating substances in the environment: estrogenic effects of pharmaceutical products. International Journal of Toxicology 1998;17:139-158.
Desbrow C, Waldock M, et al. The identification of compounds causing endocrine disruption in fish in UK rivers. Presented at the 17th Annual Meeting of the Society of Environmental Toxicology and Chemistry, Washington, DC, 1996.
Journal Articles on this Report : 3 Displayed | Download in RIS Format
| Other project views: | All 20 publications | 8 publications in selected types | All 7 journal articles |
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Foran CM, Bennett ER, Benson WH. Developmental evaluation of a potential non-steroidal estrogen: triclosan. Marine Environmental Research 2000;50(1-5):153-156. |
R827098 (1999) R827098 (2000) R827098 (2001) |
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Foran CM, Bennett ER, Benson WH. Exposure to environmentally relevant concentrations of different nonylphenol formulations in Japanese medaka. Marine Environmental Research 2000;50(1-5):135-139. |
R827098 (1999) R827098 (2000) R827098 (2001) |
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Metcalfe TL, Metcalfe CD, Kiparissis Y, Niimi AJ, Foran CM, Benson WH. Gonadal development and endocrine responses in Japanese medaka (Oryzias latipes) exposed to o,p′-DDT in water or through maternal transfer. Environmental Toxicology and Chemistry 2000;19(7):1893-1900. |
R827098 (1999) R827098 (2000) R827098 (2001) |
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Supplemental Keywords:
water, risk assessment, health effects, dose-response, metabolism, indicators, aquatic, environmental chemistry, biology, zoology, analytical, agriculture, wastewater, development, critical period, steroidogenesis, pituitary function, hatching success., RFA, Health, Scientific Discipline, PHYSICAL ASPECTS, Toxics, Geographic Area, Health Risk Assessment, Environmental Chemistry, Endocrine Disruptors - Environmental Exposure & Risk, pesticides, State, endocrine disruptors, Risk Assessments, Biochemistry, Physical Processes, Endocrine Disruptors - Human Health, Biology, predictors of reproductive toxicity, testosterone, fish, altered sexual development, endocrine disrupting chemicals, Japanese medaka, plasma hormone levels, exposure, Mississippi (MS), cellular level endocrine status, atrazine, estrogen receptors, gonad morphology, transgenerational model, concentration-effect resposnses, epidemiologic studies, animal reporductive impairment, exposure assessmentRelevant Websites:
http://www.olemiss.edu/depts/pharmacology/etrp/index.htmlProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.