Grantee Research Project Results
Final Report: Nanocavity sensor array for the isolation, detection and quantitation of engineered nanoparticles
EPA Grant Number: R834091Title: Nanocavity sensor array for the isolation, detection and quantitation of engineered nanoparticles
Investigators: Sadik, Omowunmi
Institution: The State University of New York at Binghamton
EPA Project Officer: Hahn, Intaek
Project Period: December 1, 2008 through November 30, 2011
Project Amount: $399,375
RFA: Greater Research Opportunities: Detection and Monitoring of Engineered Nanomaterials (2007) RFA Text | Recipients Lists
Research Category: Nanotechnology , Safer Chemicals
Objective:
Summary/Accomplishments (Outputs/Outcomes):
Accomplishments
- The PI started the first Gordon Conference on Environmental Nanotechnology and chaired the first meeting, which was held at the Waterville Valley Resort, Waterville, NH from May 29-June 3, 2011 (Conference program, Table 1).
- This grant has led to the development of the mass-sensitive nanosensor for the isolation and quantitative analyses of engineered fullerene (C60) nanoparticles, while excluding mixtures of structurally similar fullerenes.
- PAA membranes were successfully fabricated as flexible, free-standing membranes using phase inversion techniques. These membranes show well-defined and uniform filtration properties. The surface pore sizes were controlled by the synthesis conditions, method of desolvation and the concentrations of the polymer composition. The synthetic approach affords well controlled membranes leading to various desired sizes from less than 5nm to greater than 100nm.
- Preliminary results showed that these PAA membranes can filter quantum dots (smaller than 5nm) directly from aqueous solution at 96.78% efficiency. The standard deviation could be significantly improved at much lower concentrations of the nanoparticles (5nm).
- PAA membranes were placed on a 13mm Millipore stainless steel swinny filter holder to create a filter paper-type device. This is followed by the addition of the environmental (aqueous) samples to be filtered: Specified volume (mL) of aqueous food supplements and environmental samples containing the AgNPs or specified volume (ml) standard AgNPs stocking solutions. The AgNPs contained in the aqueous samples were trapped on the PAA membranes. The captured AgNPs were imaged and characterized by SEM and EDX techniques.
- PAA have been tested as nanofilters to isolate and remove silver nanoparticles, quantum dots and titanium dioxide particles in food supplements and environmental samples. Filtration efficiency of over 99% was recorded.
- PAA membranes were also used as a sensor for silver nanoparticles and other synthetic metal oxides. Results showed that concentration relationship in low concentration range (0.1ppm to 10ppm) with detection limit of ~50ppb.
- Filtration characteristics of the PAA membranes using the sandwich two chamber cell showed that size-dependent isolation of engineered nanoparticles of varying sizes in water and food supplements samples could be achieved based on redox current from the silver nanoparticles. The approach was tested to remove silver nanoparticles from commercial food supplements of varying particle sizes with > 99.9% removal efficiency. PAA showed excellent performance not only for isolation at sub-nanometer size range but also as a platform for detection of engineered nanoparticles.
- PAA have been tested for application in environmental matrices with significant improvements recorded over the conventional approaches for Chromium (VI), silver nanoparticles and fullerenes (Table 2).
Figure 1. Senior testing and Water collection at the BOrough of MOrrisville Waste Water Treatment
Plant Bucks County, Pennsylvania. The WWTP has annual average flow of 7.1 mgd and has
established a hydraulic capacity of 8.7 mgd.
Reductant | Intial [Cr(VI)] mM | Rate constant (k) moI-1.L.S-1 | Reaction order | Reaction (minutes) | TIme | Reference |
---|---|---|---|---|---|---|
Formic acid/PdNPs | 7.14 | 1.0878 | Pesudo-first | 5 | 25 | |
Sulfur/PdNPs | 0.40 | 0.1078 | Pesudo-first | 60 | 26 | |
1-Butanal | 11.8 | Not reported | Pesudo-first | 15 | 29 | |
Hydrogen sulfide | 0.02 | 31.9±4.2 | Pesudo-first | 70 | 30 | |
Lactic acid | 0.25 | 4.74 | Pesudo-first | 2.5 | 31 | |
PAA/PdNPs | 0.0005 | 0.2885 | Pesudo-first | 14 | This work | |
Adopted from publication # 4 which appeared in ACA Catalysis, 1, 139-146-211 |
Conclusions:
We have developed a new class of nanostructured poly(amic acid) PAA-membranes that are conductive and electroactive by preventing its imidization to polyimide, while retaining its carboxylic acid and amine functionalities. The effects of composition and microstructure on the optical and electrochemical properties of PAA hybrid composites have been studied. The uniqueness of PAA lies in its excellent physical and chemical properties: transparency, flexibility, electrical conductivity, and accessibility to forming large-area device. The membranes showed remarkable potential as sensors for engineered nanoparticles as well as for nano-filtration of the same materials from complex matrices. We have explored the use of these membranes to sense emerging contaminants such as nano-engineered fullerenes, quantum dots and nanosilver. The membranes have also been tested as filters and the possibility of ultrasensitive detection of the nanomaterials has been explored.
References:
Journal Articles on this Report : 15 Displayed | Download in RIS Format
Other project views: | All 50 publications | 15 publications in selected types | All 15 journal articles |
---|
Type | Citation | ||
---|---|---|---|
|
Du N, Wong C, Feurstein M, Sadik OA, Umbach C, Sammakia B. Flexible poly(amic acid) conducting polymers: effect of chemical composition on structural, electrochemical, and mechanical properties. Langmuir 2010;26(17):14194-14202. |
R834091 (Final) |
Exit |
|
Kikandi SN, Okello VA, Wang Q, Sadik OA, Varner KE, Burns SA. Size-exclusive nanosensor for quantitative analysis of fullerene C60. Environmental Science & Technology 2011;45(12):5294-5300. |
R834091 (Final) |
Exit Exit |
|
Mwilu SK, Aluoch AO, Miller S, Wong P, Sadik OA, Fatah AA, Arcilesi RD. Identification and quantitation of Bacillus globigii using metal enhanced electrochemical detection and capillary biosensor. Analytical Chemistry 2009;81(18):7561-7570. |
R834091 (Final) |
Exit |
|
Noah NM, Mwilu SK, Sadik OA, Fatah AA, Arcilesi RD. Immunosensors for quantifying cyclooxygenase 2 pain biomarkers. Clinica Chimica Acta 2011;412(15-16):1391-1398. |
R834091 (Final) |
Exit |
|
Noah NM, Alam S, Sadik OA. Detection of inducible nitric oxide synthase using a suite of electrochemical, fluorescence, and surface plasmon resonance biosensors. Analytical Biochemistry 2011;413(2):157-163. |
R834091 (Final) |
Exit Exit Exit |
|
Noah NM, Marcells O, Almalleti A, Lim J, Sadik OA. Metal enhanced electrochemical cyclooxygenase-2 (COX-2) sensor for biological applications. Electroanalysis 2011;23(10):2392-2399. |
R834091 (Final) |
Exit |
|
Okello VA, Du N, Deng B, Sadik OA. Environmental applications of poly(amic acid)-based nanomaterials. Journal of Environmental Nanotechnology 2011;13(5):1236-1245. |
R834091 (Final) |
Exit |
|
Omole MA, Noah N, Zhou L, Almaletti A, Sadik OA, Asemota HN, William ES, Gilchrist J. Spectroelectrochemical characterization of pain biomarkers. Analytical Biochemistry 2009;395(1):54-60. |
R834091 (Final) |
Exit Exit Exit |
|
Omole MA, Okello VA, Lee V, Zhou L, Sadik OA, Umbach C, Sammakia B. Catalytic reduction of hexavalent chromium using flexible nanostructured poly(amic acids). ACS Catalysis 2011;1(2):139-146. |
R834091 (Final) |
Exit |
|
Sadik OA. Foreword: JEM spotlight: applications of advanced nanomaterials for environmental monitoring. Journal of Environmental Monitoring 2009;11(1):25-26. |
R834091 (Final) |
Exit |
|
Sadik OA, Zhou AL, Kikandi S, Du N, Wang Q, Varner K. Sensors as tools for quantitation, nanotoxicity and nanomonitoring assessment of engineered nanomaterials. Journal of Environmental Monitoring 2009;11(10):1782-1800. |
R834091 (Final) |
Exit |
|
Sadik OA, Mwilu SK, Aluoch A. Smart electrochemical biosensors: from advanced materials to ultrasensitive devices. Electrochimica Acta 2010;55(14):4287-4295. |
R834091 (Final) |
Exit |
|
Sadik OA, Noah NM, Okello VA, Sun Z. Catalytic reduction of hexavalent chromium using palladium nanoparticles: an undergraduate nanotechnology laboratory. Journal of Chemical Education 2014;91(2):269-273. |
R834091 (Final) |
Exit |
|
Sadik OA, Aluoch AO, Zhou A. Status of biomolecular recognition using electrochemical techniques. Biosensors & Bioelectronics 2009;24(9):2749-2765. |
R834091 (Final) |
Exit |
|
Sadik OA. Anthropogenic nanoparticles in the environment. Environmental Science: Processes & Impacts 2013;15(1):19-20. |
R834091 (Final) |
Exit |
Supplemental Keywords:
Category II, nanosensors, cyclodextrin, nanomaterials, quartz crystal microbalanceProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.