Grantee Research Project Results

2002 Progress Report: Development of a Model to Predict the Bioavailability of Metals to Soil Invertebrates

EPA Grant Number: R829500C006
Subproject: this is subproject number 006 , established and managed by the Center Director under grant R829500
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Center for the Study of Metals in the Environment
Center Director: Allen, Herbert E.
Title: Development of a Model to Predict the Bioavailability of Metals to Soil Invertebrates
Investigators: Lanno, Roman
Institution: Oklahoma State University
EPA Project Officer: Hahn, Intaek
Project Period: April 1, 2002 through March 31, 2004
Project Period Covered by this Report: April 1, 2002 through March 31,2003
RFA: Targeted Research Center (2002) Recipients Lists
Research Category: Targeted Research , Hazardous Waste/Remediation

Objective:

The objective of this research project is to examine the relationship between physical and chemical characteristics of soils (e.g., organic matter, pH, cation exchange capacity) and the bioavailability, uptake, metabolism, and toxicity of zinc (Zn) and cadmium (Cd) by soil invertebrates.  Metal speciation in pore water and the invertebrate gastrointestinal system and tissues also will be determined to develop a Biotic Ligand Model (BLM) for soil invertebrates, which would enhance our ability to predict the toxicity of metals in soil systems.  Research during this past year has focused on the development of a method for the fractionation of earthworms to provide a tool for examining the partitioning of metals within a soil invertebrate species.  This is the first step in understanding metal metabolism in various organisms to determine which fraction of metal in the organism best represents a biotic ligand associated with a toxic response.

Progress Summary:

Preliminary findings of this research project and continuing collaborative studies with Willie Peijnenburgh (RIVM, The Netherlands) and Martina Vijver (Vrije Universiteit, Amsterdam) show that metal distribution within the earthworm body is different for essential and nonessential metals.  Cd is predominantly stored in the metal fraction associated with the protein fraction, and induction of this fraction was shown to occur upon exposure to Cd.  Zn is more evenly distributed over the various metal fractions, and induction of the protein fraction was not observed following exposure to Zn.  Significantly increased Cd levels were found in the metal fraction associated with the cell tissue and the cell membranes following feeding of the litter feeding worm Lumbricus rubellus with Cd-contaminated leaf material in soils that contained high metal levels (total concentration) and low Cd concentrations in the pore water.  Exposure of the nonlitter feeding Acosmetia caliginosa did not lead to an increase in Cd levels, whereas Zn levels did not increase in either species.  This is probably due to regulation of internal Zn levels in the worms.  These findings are consistent with metal levels found in field-collected worms, and internal Cd and Zn distribution also agrees with laboratory observations.  Following exposure of worms to soils spiked with metal salts, a marked increase in levels of metals associated with the protein fraction was observed.  These observations allow for distinguishing the kinetics of elimination of the metal fractions associated with the protein fraction and the cell membranes/cell tissue.  These experiments currently are in progress.

Future Activities:

We will continue to examine the relationship between physical and chemical characteristics of soils and the bioavailability, uptake, metabolism, and toxicity of Zn and Cd by soil invertebrates.

Journal Articles on this Report : 3 Displayed | Download in RIS Format

Publications Views

Other subproject views:	All 3 publications	3 publications in selected types	All 3 journal articles
Other center views:	All 4 publications	4 publications in selected types	All 4 journal articles

Publications

Type	Citation	Sub Project	Document Sources
Journal Article	Field LJ, Macdonald DD, Norton SB, Ingersoll CG, Severn CG, Smorong D, Lindskoog R. Predicting amphipod toxicity from sediment chemistry using logistic regression models. Environmental Toxicology and Chemistry 2002;21(9):1993-2005.	R829500 (2002) R829500C001 (2002) R829500C002 (2002) R829500C003 (2002) R829500C004 (2002) R829500C005 (2002) R829500C006 (2002) R829500C007 (2002)	Abstract from PubMed
Journal Article	Paquin PR, Gorsuch JW, Apte S, Batley GE, Bowles KC, Campbell PGC, Delos CG, Di Toro DM, Dwyer RL, Galvez F, Gensemer RW, Goss GG, Hogstrand C, Janssen CR, McGeer JC, Naddy RB, Playle RC, Santore RC, Schneider U, Stubblefield WA, Wood CM, Wu KB. The biotic ligand model: a historical overview. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2002;133(1-2):3-35.	R829500 (2002) R829500C001 (2002) R829500C002 (2002) R829500C003 (2002) R829500C004 (2002) R829500C005 (2002) R829500C006 (2002) R829500C007 (2002)	Abstract from PubMed Full-text: Science Direct Full Text Exit Other: Science Direct PDF Exit
Journal Article	Santore RC, Mathew R, Paquin PR, DiToro DM. Application of the biotic ligand model to predicting zinc toxicity to rainbow trout, fathead minnow, and Daphnia magna. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2002;133(1-2):271-285.	R829500 (2002) R829500C001 (2002) R829500C002 (2002) R829500C003 (2002) R829500C004 (2002) R829500C005 (2002) R829500C006 (2002) R829500C007 (2002)	Abstract from PubMed Full-text: Science Direct Full Text Exit Other: Science Direct PDF Exit

Supplemental Keywords:

bioavailability, metals, soil, soil invertebrates, toxicity, metabolism, metal uptake, cadmium, Cd, zinc, Zn, metal speciation, Biotic Ligand Model, BLM,, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Waste, Geochemistry, Hazardous Waste, Environmental Monitoring, Ecological Risk Assessment, Hazardous, fate and transport , modeling, remediation, aquatic ecosystems, mobility of contaminants, bioaccumulation, metal contamination

Relevant Websites:

http://www.ce.udel.edu/CSME/Index.html Exit

Progress and Final Reports:

Original Abstract

Final

Main Center Abstract and Reports:

R829500    Center for the Study of Metals in the Environment

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829500C001 Role of Dietary Exposure for Bioaccumulation and Toxicity of Metals in Aquatic Ecosystems Affected by Mining
R829500C002 The Role of Organic Matter and Metal Oxides in the Retention of Trace Metals by Soil and Suspended Particles
R829500C003 Developing a Model to Predict the Persistence of Metals in Aquatic Environments
R829500C004 Effects of Dietary Metal Exposure on Fish and Aquatic Invertebrates
R829500C005 Aquatic Toxicity and Exposure Assessment
R829500C006 Development of a Model to Predict the Bioavailability of Metals to Soil Invertebrates
R829500C007 Bioaccumulation and Toxicity of Dietborne Particulate Metals to Benthic Invertebrates