Grantee Research Project Results
Final Report: Development and application of a fiber optic array system for detection and enumeration of potentially toxic cyanobacteria
EPA Grant Number: R833828Title: Development and application of a fiber optic array system for detection and enumeration of potentially toxic cyanobacteria
Investigators: Anderson, Donald M. , Carmichael, Wayne W
Institution: Woods Hole Oceanographic Institution
EPA Project Officer: Aja, Hayley
Project Period: June 1, 2008 through May 31, 2011 (Extended to May 31, 2013)
Project Amount: $508,494
RFA: Development and Evaluation of Innovative Approaches for the Quantitative Assessment of Pathogens and Cyanobacteria and Their Toxins in Drinking Water (2007) RFA Text | Recipients Lists
Research Category: Drinking Water , Water
Objective:
The overall project goal was to adapt and validate a rapid and accurate optical fiber-based technology for cyanoHAB cell detection and enumeration in both laboratory and field settings. Specific aims were to: (1) design ribosomal RNA (rRNA) signal and capture probes for the three most important toxic cyanobacteria (Microcystis, Cylindrospermopsis, and Anabaena) using published sequences; (2) design and test a second probe pair for each species, to incorporate redundancy into the array; (3) test these probes in the fiber-optic array format and determine detection limits, specificity, and dynamic range; (4) refine hybridization conditions to reduce processing time; (5) develop procedures to analyze multiple cyanoHAB species simultaneously using a single fiber bundle in a multiplexed format and validate it using mixed cultures and spiked and unspiked field samples; (6) work with individuals and agencies responsible for fresh- and brackish water management to determine desired detection limits, precision, new cyanobacteria species for future probe design, and operational characteristics for the assay and instrumentation that would be developed around it; and (7) prepare a detailed protocol for sample handling and processing for use with this method.
Summary/Accomplishments (Outputs/Outcomes):
Conclusions:
References:
Kibbe WA (2007). "OligoCalc: an online oligonucleotide properties calculator." Nucleic Acids Res. 35: May 25.
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 12 publications | 1 publications in selected types | All 1 journal articles |
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Type | Citation | ||
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Steinberg M, First M, Lemieux E, Drake L, Nelson B, Kulis D, Anderson D, Welshmeyer N, Herring P. Comparison of techniques used to count single-celled viable phytoplankton. JOURNAL OF APPLIED PHYCOLOGY 2012;24(4):751-758. |
R833828 (Final) |
Exit Exit |
Supplemental Keywords:
Health effects, ecological effects, human health, toxics, bacteria, ecosystem, aquatic, environmental chemistry, biology, ecology, genetics, limnology, monitoring, analytical, northeast, central, northwest, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Water, Environmental Chemistry, Health Risk Assessment, Environmental Monitoring, Environmental Engineering, Drinking Water, microbial contamination, microbial risk assessment, monitoring, real time analysis, gene microarray assay, aquatic organisms, other - risk assessment, early warning, drinking water contaminants, drinking water systemProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.