Grantee Research Project Results
Final Report: Bioavailability and Fates of CdSe and TiO2 Nanoparticles in Eukaryotes and Bacteria
EPA Grant Number: R833323Title: Bioavailability and Fates of CdSe and TiO2 Nanoparticles in Eukaryotes and Bacteria
Investigators: Holden, Patricia , Nadeau, Jay L. , Stucky, Galen
Institution: University of California - Santa Barbara , McGill University
EPA Project Officer: Aja, Hayley
Project Period: May 15, 2007 through May 14, 2010
Project Amount: $399,986
RFA: Exploratory Research: Nanotechnology Research Grants Investigating Environmental and Human Health Effects of Manufactured Nanomaterials: a Joint Research Solicitation-EPA, NSF, NIOSH, NIEHS (2006) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Nanotechnology , Safer Chemicals
Objective:
The objectives of this project were to research interactions of CdSe quantum dots and TiO2 nanoparticles with bacteria and eukaryotic cells including cellular uptake, extra- and intracellular particle or conjugate transformations, and effects on cells, plus to relate uptake, processing and effects to nanoparticle type and characteristics. Significant progress was made in each of these issues.
Summary/Accomplishments (Outputs/Outcomes):
Conclusions:
In a three year multi-investigator, multidisciplinary study of fundamental interactions of QDs and nanoscale TiO2 with bacterial and mammalian cells, new QD-based approaches in cell visualization were developed. These approaches are predicated upon intimate interaction between nanoparticles and cells which was revealed to result in toxicity that was QD-type, strain, and light-dependent. Specific forms of ROS were quantified as the agents of cell damage, and electron transfer from cells to QDs with concomitant ROS formation and cell damage were observed. Light conditions were not necessary. Organic conjugates that are effective electron transfer agents will affect ROS production and thus toxicity, differently for CdSe vs. CdTe QDs. Uptake of QDs into cells is efficient, and can result in biomagnification into protozoan predators. Membrane associations are not limited to QDs: nanoscale TiO2 also associates with bacterial membranes, resulting in disagglomeration of previously agglomerated TiO2. Such association may afford the particle-size-dependent growth inhibition of TiO2 in the dark observed for one bacterial strain. The possibility that electron transfer and ROS formation can explain nanoscale TiO2 toxicity to bacteria in the dark is unexplored in this study, but is motivated for future study by the results of this research. Overall, this research provides new and important insights into mechanisms of select nanomaterial toxicity to bacteria. This research suggests that nanoparticles are frequently growth-inhibitory, particularly under conditions that promote direct cell contact. While not directly studied in this research, there may be broader implications to bacterially-driven processes in the environment as most biogeochemical processes in nature are catalyzed through bacterial population growth as was mostly studied in this research.
Journal Articles on this Report : 13 Displayed | Download in RIS Format
Other project views: | All 44 publications | 15 publications in selected types | All 13 journal articles |
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Clarke S, Koshy S, Zhang J, Cohen N, Nadeau J. Power and wavelength dependence of photoenhancement in (CdSe)ZnS-dopamine in aqueous solution and live cells. Zeitschrift fur Physikalische Chemie 2008;222(5-6):851-863. |
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Clarke S, Mielke RE, Neal A, Holden P, Nadeau JL. Bacterial and mineral elements in an arctic biofilm: a correlative study using fluorescence and electron microscopy. Microscopy and Microanalysis 2010;16(2):153-165. |
R833323 (Final) |
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Conti JA, Killpack K, Gerritzen G, Huang L, Mircheva M, Delmas M, Harthorn BH, Appelbaum RP, Holden PA. Health and safety practices in the nanomaterials workplace: results from an international survey. Environmental Science & Technology 2008;42(9):3155-3162. |
R833323 (2008) R833323 (Final) |
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Cooper DR, Suffern D, Carlini L, Clarke SJ, Parbhoo R, Bradforth SE, Nadeau JL. Photoenhancement of lifetimes in CdSe/ZnS and CdTe quantum dot-dopamine conjugates. Physical Chemistry Chemical Physics 2009;11(21):4298-4310. |
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Cooper DR, Dimitrijevic NM, Nadeau JL. Photosensitization of CdSe/ZnS QDs and reliability of assays for reactive oxygen species production. Nanoscale 2010;2(1):114-121. |
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Dumas EM, Ozenne V, Mielke RE, Nadeau JL. Toxicity of CdTe quantum dots in bacterial strains. IEEE Transactions on Nanobioscience 2009;8(1):58-64. |
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Dumas E, Gao C, Suffern D, Bradforth SE, Dimitrijevic NM, Nadeau JL. Interfacial charge transfer between CdTe quantum dots and gram negative vs gram positive bacteria. Environmental Science & Technology 2010;44(4):1464-1470. |
R833323 (Final) R833339 (Final) |
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Ehrhardt CJ, Haymon RM, Sievert SM, Holden PA. An improved method for nanogold in situ hybridization visualized with environmental scanning electron microscopy. Journal of Microscopy 2009;236(1):5-10. |
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Horst AM, Neal AC, Mielke RE, Sislian PR, Suh WH, Madler L, Stucky GD, Holden PA. Dispersion of TiO2 nanoparticle agglomerates by Pseudomonas aeruginosa. Applied and Environmental Microbiology 2010;76(21):7292-7298. |
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Kaats AJ, Galiana HL, Nadeau JL. Standardizing the atomic description, axis and centre of biological ion channels. Journal of Neuroscience Methods 2007;165(1):135-143. |
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Khatchadourian R, Bachir A, Clarke SJ, Heyes CD, Wiseman PW, Nadeau JL. Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells. Journal of Biomedicine & Biotechnology 2007;2007:70145 (10 pp.). |
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Priester JH, Stoimenov PK, Mielke RE, Webb SM, Ehrhardt C, Zhang JP, Stucky GD, Holden PA. Effects of soluble cadmium salts versus CdSe quantum dots on the growth of planktonic Pseudomonas aeruginosa. Environmental Science & Technology 2009;43(7):2589-2594. |
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Werlin R, Priester JH, Mielke RE, Kramer S, Jackson S, Stoimenov PK, Stucky GD, Cherr GN, Orias E, Holden PA. Biomagnification of cadmium selenide quantum dots in a simple experimental microbial food chain. Nature Nanotechnology 2011;6(1):65-71. |
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Supplemental Keywords:
water, soil, chemical transport, effects, ecological effects, bioavailability, metabolism, dose response, organism, population, chemicals, toxics, metals, heavy metals, oxidants, bacteria, terrestrial, aquatic, environmental microbiology, biofilms, chemical analysis, nanotechnology, DNA , RFA, Scientific Discipline, Sustainable Industry/Business, treatment, control, Analytical Chemistry, Biochemistry, Chemistry and Materials Science, Environmental Chemistry, Environmental Engineering, New/Innovative technologies, Sustainable Environment, Technology, Technology for Sustainable Environment, DNA damage, biofilm, engineering, environmental sustainability, environmentally applicable nanoparticles, heavy metal sequestration, innovative technologies, innovative technology, nanotechnology, quantum dots, semiconductor nanocrystals
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.