Grantee Research Project Results
2007 Progress Report: Development of Molecular Biomarkers to Measure Environmentally Induced Immune Responses
EPA Grant Number: R832947Title: Development of Molecular Biomarkers to Measure Environmentally Induced Immune Responses
Investigators: Miller, Lisa A. , Gilliland, Frank D. , Margolis, Helene , Gern, James , Joad, Jesse , Abel, Kristina
Current Investigators: Miller, Lisa A. , Gilliland, Frank D. , Abel, Kristina , Margolis, Helene , Gern, James , Joad, Jesse
Institution: University of California - Davis
EPA Project Officer: Callan, Richard
Project Period: June 15, 2006 through June 14, 2009 (Extended to December 14, 2011)
Project Period Covered by this Report: June 15, 2007 through June 14,2008
Project Amount: $712,423
RFA: Early Indicators of Environmentally Induced Disease (2004) RFA Text | Recipients Lists
Research Category: Human Health
Objective:
- Develop a high-throughput quantitative RT-PCR assay for leukocyte cluster of differentiation markers to measure key immune cell populations associated with allergy and asthma in small biological samples.
- Develop a high-throughput quantitative RT-PCR assay for key cytokine markers associated with allergy and asthma to measure immune cell function in small biological samples.
Approach:
In this proposal, we will develop a panel of oligonucleotide primers and probes for real-time quantitative PCR analysis of immune cell phenotype and function. The significance of each immune biomarker in the context of environmentally induced allergic airways disease will subsequently be determined by evaluating peripheral blood and airway cells obtained from an infant rhesus monkey model of environmentally induced asthma.
Progress Summary:
Expected Results:
This study will generate a panel of sensitive molecular biomarkers to measure environmentally induced changes in systemic and local immune responses within small biological samples. Once tested and characterized, these reagents can be immediately incorporated as a part of the National Children’s Study to investigate how environmental exposure to air pollutants can modulate the immune system during early childhood development.
Future Activities:
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 6 publications | 4 publications in selected types | All 4 journal articles |
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Type | Citation | ||
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Chou DL, Gerriets JE, Schelegle ES, Hyde DM, Miller LA. Increased CCL24/eotaxin-2 with postnatal ozone exposure in allergen-sensitized infant monkeys is not associated with recruitment of eosinophils to airway mucosa. Toxicology and Applied Pharmacology 2011;257(3):309-318. |
R832947 (2007) R832947 (2009) R832947 (Final) |
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Supplemental Keywords:
immunology, ozone, indoor air, health effects, susceptibility, biomarkers, allergy, asthma, RFA, Health, Scientific Discipline, PHYSICAL ASPECTS, Air, HUMAN HEALTH, particulate matter, Environmental Chemistry, Health Risk Assessment, Risk Assessments, Susceptibility/Sensitive Population/Genetic Susceptibility, Allergens/Asthma, Environmental Monitoring, Physical Processes, Health Effects, genetic susceptability, ambient air quality, atmospheric particulate matter, particulates, asthma triggers, sensitive populations, asthma, air toxics, atmospheric particles, chemical characteristics, ambient air monitoring, health risks, airborne particulate matter, asthma indices, environmental risks, exposure, second hand smoke, airway disease, airway inflammation, air pollution, aerosol composition, atmospheric aerosol particles, human exposure, airborne pollutants, inhalation, human susceptibility, allergic response, tobacco smoke
Progress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.