Grantee Research Project Results
A Hybrid Pathogen Detection System
EPA Contract Number: EPD05044Title: A Hybrid Pathogen Detection System
Investigators: Aguilar, Zoraida P.
Small Business: Vegrandis Inc.
EPA Contact: Richards, April
Phase: I
Project Period: March 1, 2005 through August 31, 2005
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (2005) RFA Text | Recipients Lists
Research Category: SBIR - Water and Wastewater , Watersheds , Small Business Innovation Research (SBIR)
Description:
Cryptosporidium parvum, a protozoan parasite that invades the gastrointestinal system, is a serious threat to the Nation's water supply. Assays for pathogen using self-contained microelectrochemical detection are desirable because precise detection can be performed with simple instrumentation on colored and turbid samples, minimizing pretreatment procedures. In addition, electrochemical detection immediately adjacent to surface-bound assay components provide rapid signals even for very low analyte concentrations based on short transport distances for reporter molecules.
Phase I of this research project involves antibody capture coupled with a mRNA-hybridization assay and electrochemical detection analysis of C. parvum oocysts. The studies are expected to demonstrate improvement over U.S. Environmental Protection Agency (EPA) Methods 1623 and 1622 in several respects.
Vegrandis, LLC's proposed approach with twofold specificity has the potential to achieve rapid, onsite detection of ultra-low concentrations from large sample volumes with minimal or limited sample filtration or preparation. The detection of C. parvum oocysts from water samples will be used to demonstrate the performance of the approach. Success of this project will lead to the development of devices that will not only allow better, less expensive, and timely quality assurance and control of water supplies, but also will provide opportunities to study the fate of C. parvum in the environment from various sources to water intake sites as a function of weather and season. Such extensive and routine studies are not practical with current EPA methods of analysis.
Supplemental Keywords:
small business, SBIR, hybrid pathogen detection system, drinking water, Cryptosporidium parvum, C. parvum, electrochemical detection, analyte, oocyst, mRNA-hybridization assay, EPA, RFA, Scientific Discipline, Ecosystem Protection/Environmental Exposure & Risk, PHYSICAL ASPECTS, Water, Health Risk Assessment, Physical Processes, Environmental Chemistry, Monitoring/Modeling, Biochemistry, Drinking Water, microbial contamination, mRNA hybridization assay, assays, Safe Drinking Water, water quality, water quality parameters, microbial risk assessment, fecal contamination, monitoring, pathogens, drinking water system, drinking water contaminants, exposure, other - risk assessment, waterborne disease, microelectrochemical detection, cryptosporidium parvum oocysts, cryptosporidium , public health, drinking water distribution system, immunofluorescent assayProgress and Final Reports:
SBIR Phase II:
A Hybrid Pathogen Detection System | Final ReportThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.