Grantee Research Project Results
2005 Progress Report: Pulmonary Biomarkers Based on Alterations in Protein Expression Following Exposure to Arsenic
EPA Grant Number: R832095Title: Pulmonary Biomarkers Based on Alterations in Protein Expression Following Exposure to Arsenic
Investigators: Lantz, R. Clark , Boitano, Scott A. , Burgess, Jefferey L.
Institution: University of Arizona
EPA Project Officer: Aja, Hayley
Project Period: January 8, 2005 through January 16, 2009
Project Period Covered by this Report: January 8, 2005 through January 7,2009
Project Amount: $731,453
RFA: Application of Biomarkers to Environmental Health and Risk Assessment (2004) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation
Objective:
Exposure to arsenic (As) has been linked to lung cancer. Environmental exposure to these metals will result in multiple adverse effects, which can be characterized through evaluation of alterations in protein expression. We will evaluate such alterations as biomarkers of exposure and effect prior to the development of cancer. This study will use the technology of proteomics to evaluate and identify biomarkers of chronic environmental exposure to As by evaluating large numbers of proteins simultaneously. We will compare alterations in protein expression in exposed human populations in Arizona, human cell lines, and in vivo rodent studies. Patterns of alterations in protein expression, both common and unique to these different cell types, will be identified. These will be correlated with alterations in DNA oxidation in induced sputum from the lung, which we have recently found to be related to the levels of arsenic exposure.
Progress Summary:
- Arsenic alters wound repair, both in vitro and in vivo
- Altered calcium signaling, especially through purinergic receptors
- Altered epithelial junctional proteins and function
Future Activities:
We are continuing to analyze data and finish manuscripts that will soon be submitted for review. At least four additional manuscripts will be submitted in the next several months. These include:
Journal Articles on this Report : 5 Displayed | Download in RIS Format
Other project views: | All 18 publications | 7 publications in selected types | All 7 journal articles |
---|
Type | Citation | ||
---|---|---|---|
|
Burgess JL, Meza MM, Josyula AB, Poplin GS, Kopplin MJ, McClellen HE, Sturup S, Lantz RC. Environmental arsenic exposure and urinary 8-OHdG in Arizona and Sonora. Clinical Toxicology 2007;45(5):490-498. |
R832095 (2005) R832095 (2007) |
Exit |
|
Josyula AB, Poplin GS, Kurzius-Spencer M, McClellen HE, Kopplin MJ, Sturup S, Lantz RC, Burgess JL. Environmental arsenic exposure and sputum metalloproteinase concentrations. Environmental Research 2006;102(3):283-290. |
R832095 (2005) R832095 (2006) R832095 (2007) |
Exit Exit |
|
Lantz RC, Hays AM. Role of oxidative stress in arsenic-induced toxicity. Drug Metabolism Reviews 2006;38(4):791-804. |
R832095 (2005) R832095 (2006) R832095 (2007) |
Exit |
|
Lantz RC, Lynch BJ, Boitano S, Poplin GS, Littau S, Tsaprailis G, Burgess JL. Pulmonary biomarkers based on alterations in protein expression after exposure to arsenic. Environmental Health Perspectives 2007;115(4):586-591. |
R832095 (2005) R832095 (2006) R832095 (2007) |
|
|
Olsen CE, Liguori AE, Zong Y, Lantz RC, Burges JL, Boitano S. Arsenic upregulates MMP-9 and inhibits wound repair in human airway epithelial cells. American Journal of Physiology-Lung Cellular and Molecular Physiology 2008;295(2):L293-302. |
R832095 (2005) R832095 (2007) |
Exit Exit |
Supplemental Keywords:
heavy metals, human health, animal, cellular, biomarkers, induced sputum, bronchial epithelial cells , Health, PHYSICAL ASPECTS, RFA, Scientific Discipline, Water, Arsenic, Biochemistry, Environmental Chemistry, Hazardous Waste, Health Risk Assessment, Physical Processes, Risk Assessments, arsenic exposure, bioaccumulation, biogeochemistry, biomarker measurements, contaminant transport, contaminated sediments, exposure, RFA, Health, Scientific Discipline, Water, POLLUTANTS/TOXICS, Environmental Chemistry, Health Risk Assessment, Arsenic, Risk Assessments, Hazardous Waste, Biochemistry, Water Pollutants, contaminated sediments, contaminant transport, risk management, biogeochemistry, arsenic exposure, bioaccumulationProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.