Grantee Research Project Results
1999 Progress Report: Bioavailability & Risk Assessment of Complex Mixtures
EPA Grant Number: R825408Title: Bioavailability & Risk Assessment of Complex Mixtures
Investigators: Donnelly, Kirby C. , Reeves, William
Current Investigators: Donnelly, Kirby C. , Safe, Stephen H. , Autenrieth, R. L. , McDonald, T. J. , Reeves, William , Huebner, Henry J.
Institution: Texas A & M University
EPA Project Officer: Hahn, Intaek
Project Period: November 15, 1996 through November 14, 1999 (Extended to April 15, 2000)
Project Period Covered by this Report: November 15, 1998 through November 14, 1999
Project Amount: $443,997
RFA: Environmental Fate and Treatment of Toxics and Hazardous Wastes (1996) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management , Safer Chemicals
Objective:
The proposed research is designed to develop an accurate methodology for estimating risk and bioaccessibility of complex mixtures in soil at uncontrolled hazardous waste sites. The three major objectives are to: (1) develop a protocol for preparation of a water soluble fraction (WSF) as a method for estimating residual contaminant concentrations that are protective of human health and the environment; (2) develop a quantitative toxicokinetic model using genotoxicity and enzyme induction assays to interpret the interactions of complex mixtures of polycyclic aromatic hydrocarbons (PAHs) and halogenated aromatic hydrocarbons (HAHs); and (3) validate the WSF protocol by analysis and risk assessment of soils from four Superfund sites.Progress Summary:
The results of the initial WSF experiments have been compiled and prepared for submission to Environmental Toxicology and Chemistry. The paper should be submitted in the coming weeks.The five fractions isolated from a complex PAH mixture have been analyzed in the Salmonella/microsome assay and the Chicken Embryo Screening Test; they currently are being run in the Gap Junction Intercellular Communication Assay. The first two assays revealed that toxic equivalency factors, as forwarded by Nisbet and LaGoy (1992), do not predict the relative toxicity of the fractions. Additionally, the relative potencies forwarded by the U.S. Environmental Protection Agency (EPA) do not predict relative potencies. The sum of the total carcinogenic PAHs present seems to be the best indicator of toxicity at this time. An additional assay examining the cytochrome P450 induction potential of the fractions is planned for the coming months.
The urine samples from the coal tar contaminated soil feeding study have been analyzed. The results indicate that aging of the soil had no significant impact on the rats' uptake and excretion of pyrene from the soil. Results of Kd measurements and desorption experiments with the soils indicated similar results. Analyses of the livers will begin shortly.
Sediment samples contaminated with PAHs from different sources were collected in July 1999, and experiments to determine the partitioning and desorption behavior of the PAHs are under way.
Future Activities:
The final bioassays with the fractions will be completed by this spring. Analyses of the liver samples from the feeding study should be completed within the upcoming weeks. Finally, desorption and partitioning measurements with the PAH-contaminated sediments from Superfund sites are scheduled to be completed before mid-April 2000. The data from these studies will be used to produce three additional manuscripts for submission to technical journals.Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 8 publications | 4 publications in selected types | All 4 journal articles |
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Type | Citation | ||
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Bordelon NR, Donnelly KC, King LC, Wolf DC, Reeves WR, George SE. Bioavailability of the genotoxic components in coal tar contaminated soils in Fischer 344 rats. Toxicological Sciences 2000;56(1):37-48. |
R825408 (1998) R825408 (1999) |
not available |
Supplemental Keywords:
bioaccessibility, complex mixtures, polycyclic aromatic hydrocarbons, PAHs, halogenated aromatic hydrocarbons, toxicity, toxic equivalency factor, remediation, hazardous waste sites., RFA, Health, Scientific Discipline, Toxics, Waste, Water, Ecosystem Protection/Environmental Exposure & Risk, Bioavailability, Contaminated Sediments, Remediation, Geochemistry, Environmental Chemistry, Health Risk Assessment, pesticides, Chemistry, Risk Assessments, chemical mixtures, Fate & Transport, complex mixtures, risk assessment, fate and transport, cumulative risk, fate, coal gasification sites, Superfund sites, effects assessment, risk assessment techniques, contaminant transport, soil sediment, acceptable residue levels, contaminated sediment, complex mitures, sediment transport, transport contaminants, geotoxicity, PAH metal mixtures, adverse human health affects, chemical contaminants, soils, contaminated soil, PAH, human exposure, toxicity, characterizing chemical mixtures, environmental transport and fate, public health, furans, environmental toxicant, Toxic Equivalency Factor, hazardous materials exposure, assessment methods, refinery sites, vadose zone, hazardous waste sites, dioxins, exposure assessment, environmental chemicals, toxicokineticsProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.