Grantee Research Project Results

Final Report: Plant Genes and Agrobacterium T-DNA Integration

EPA Grant Number: R829479C001
Subproject: this is subproject number 001 , established and managed by the Center Director under grant R829479
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: Duke University Center for Environmental Implications of NanoTechnology
Center Director: Wiesner, Mark R.
Title: Plant Genes and Agrobacterium T-DNA Integration
Investigators: Gelvin, Stanton B.
Institution: Purdue University
EPA Project Officer: Aja, Hayley
Project Period: July 1, 2002 through June 30, 2004
RFA: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program (2001) RFA Text | Recipients Lists
Research Category: Targeted Research , Hazardous Waste/Remediation

Objective:

The objectives of this research project were to:

identify and characterize Arabidopsis genes that are involved in Agrobacterium tumefaciens T-DNA integration;
and initiate a search for Arabidopsis mutants that are hyper-susceptible to Agrobacterium transformation (hat mutants).

The expectation was that the characterization and manipulation of these “integration” genes would enhance Agrobacterium-mediated transformation of recalcitrant crop species.

Summary/Accomplishments (Outputs/Outcomes):

The following conclusions were drawn from the study:

Some chromatin mutants are rat mutants (although many are not).
Over expression of some histone genes other than HTA1 can enhance Agrobacterium-mediated transformation.
Various Arabidopsis histone H2A genes have differing patterns of expression.
The investigators have been able to identify Arabidopsis hat mutants from a T-DNA activation-tag mutant collection.

Journal Articles on this Report : 8 Displayed | Download in RIS Format

Publications Views
Other subproject views:	All 56 publications	8 publications in selected types	All 8 journal articles
Other center views:	All 212 publications	49 publications in selected types	All 45 journal articles

Publications
Type	Citation	Sub Project	Document Sources
Journal Article	Gaspar YM, Nam J, Schultz CJ, Lee L-Y, Gilson PR, Gelvin SB, Bacic A. Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of agrobacterium transformation. Plant Physiology 2004;135(4):2162-2171.	R829479 (2006) R829479 (Final) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: Plant Physiology-Full-Text Exit Abstract: Plant Physiology-Abstract Exit Other: Plant Physiology-PDF Exit
Journal Article	Gelvin SB. Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiology and Molecular Biology Reviews 2003;67(1):16-37.	R829479 (2006) R829479 (Final) R829479C001 (2003) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: ASM-Full-Text Exit Abstract: ASM-Abstract Exit Other: ASM-PDF Exit
Journal Article	Gelvin SB. Improving plant genetic engineering by manipulating the host. Trends in Biotechnology 2003;21(3):95-98.	R829479 (2006) R829479 (Final) R829479C001 (2003) R829479C001 (Final)	Abstract from PubMed Full-text: ScienceDirect-Full Text HTML Exit Abstract: Cell-Abstract Exit Other: ScienceDirect-PDF Exit
Journal Article	Tao Y, Rao PK, Bhattacharjee S, Gelvin SB. Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2. Proceedings of the National Academy of Sciences of the United States of America 2004;101(14):5164-5169.	R829479 (2006) R829479 (Final) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: PNAS-Full-Text Exit Abstract: PNAS-Abstract Exit Other: PNAS-PDF Exit
Journal Article	Tian L, Wang JL, Fong MP, Chen M, Cao HB, Gelvin SB, Chen ZJ. Genetic control of developmental changes induced by disruption of Arabidopsis histone deacetylase 1 (AtHD1) expression. Genetics 2003;165(1):399-409.	R829479 (2006) R829479 (Final) R829479C001 (2003) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: Genetics-Full-Text Exit Abstract: Genetics-Abstract Exit Other: Genetics-PDF Exit
Journal Article	van Attikum H, Bundock P, Overmeer RM, Lee L-Y, Gelvin SB, Hooykaas PJJ. The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA. Nucleic Acids Research 2003;31(14):4247-4255.	R829479 (2006) R829479 (Final) R829479C001 (2003) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: Nucleic Acids Research-Full-Text Exit Abstract: Nucleic Acids Research-Abstract Exit Other: Nucleic Acids Research-PDF Exit
Journal Article	Yi HC, Mysore KS, Gelvin SB. Expression of the Arabidopsis histone H2A-1 gene correlates with susceptibility to Agrobacterium transformation. The Plant Journal 2002;32(3):285-298.	R829479 (2006) R829479 (Final) R829479C001 (Final)	Abstract from PubMed Full-text: Wiley-PDF Exit Abstract: Wiley-Abstract Exit
Journal Article	Zhu Y, Nam J, Humara JM, Mysore KS, Lee L-Y, Cao HB, Valentine L, Li JL, Kaiser AD, Kopecky AL, Hwang H-H, Bhattacharjee S, Rao PK, Tzfira T, Rajagopal J, Yi HC, Veena, Yadav BS, Crane YM, Lin K, Larcher Y, Gelvin MJK, Knue M, Ramos C, Zhao X, Davis SJ, Kim S-I, Ranjith-Kumar CT, Choi Y-J, Hallan VK, Chattopadhyay S, Sui X, Ziemienowicz A, Matthysse AG, Citovsky V, Hohn B, Gelvin SB. Identification of Arabidopsis rat mutants. Plant Physiology 2003;132(2):494-505.	R829479 (2006) R829479 (Final) R829479C001 (2003) R829479C001 (Final)	Full-text from PubMed Abstract from PubMed Associated PubMed link Full-text: Plant Physiology-Full-Text Exit

Supplemental Keywords:

Agrobacterium tumefaciens, plant genetic transformation, T-DNA, histone, transgenic plants, chromatin, T-DNA integration, rat mutants, hat mutants, sustainable industry/business, agricultural engineering, environmental engineering, genetics, geochemistry, new/innovative technologies, Arabidopsis, bioenergy, bioengineering, biotechnology, cloning, plant biotechnology, plant genes,, Scientific Discipline, Sustainable Industry/Business, Genetics, New/Innovative technologies, Environmental Engineering, Agricultural Engineering, agrobacterium, bioengineering, T-DNA, transgenic plants, plant genes, Arabidopsis, biotechnology, remediation, plant biotechnology, cloning

Relevant Websites:

http://www.bio.purdue.edu/about/faculty/gelvin/gelvinweb/gelvin.html Exit
http://www.chromdb.org/v3/test2.html Exit
http://atidb.cshl.org Exit
http://www.cpbr.org Exit

Progress and Final Reports:

Original Abstract

2003 Progress Report

Main Center Abstract and Reports:

R829479 Duke University Center for Environmental Implications of NanoTechnology

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829479C001 Plant Genes and Agrobacterium T-DNA Integration
R829479C002 Designing Promoters for Precision Targeting of Gene Expression
R829479C003 aka R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C004 Negative Sense Viral Vectors for Improved Expression of Foreign Genes in Insects and Plants
R829479C005 Development of Novel Plastics From Agricultural Oils
R829479C006 Conversion of Paper Sludge to Ethanol
R829479C007 Enhanced Production of Biodegradable Plastics in Plants
R829479C008 Engineering Design of Stable Immobilized Enzymes for the Hydrolysis and Transesterification of Triglycerides
R829479C009 Discovery and Evaluation of SNP Variation in Resistance-Gene Analogs and Other Candidate Genes in Cotton
R829479C010 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals
R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C012 High Strength Degradable Plastics From Starch and Poly(lactic acid)
R829479C013 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C014 Identification of Receptors of Bacillus Thuringiensis Toxins in Midguts of the European Corn Borer
R829479C015 Coordinated Expression of Multiple Anti-Pest Proteins
R829479C016 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C017 Molecular Improvement of an Environmentally Friendly Turfgrass
R829479C018 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals. II.
R829479C019 Transgenic Plants for Bioremediation of Atrazine and Related Herbicides
R829479C020 Root Exudate Biostimulation for Polyaromatic Hydrocarbon Phytoremediation
R829479C021 Phytoremediation of Heavy Metal Contamination by Metallohistins, a New Class of Plant Metal-Binding Proteins
R829479C022 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C023 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C024 Development of Vectors for the Stoichiometric Accumulation of Multiple Proteins in Transgenic Crops
R829479C025 Chemical Induction of Disease Resistance in Trees
R829479C026 Development of Herbicide-Tolerant Hardwoods
R829479C027 Environmentally Superior Soybean Genome Development
R829479C028 Development of Efficient Methods for the Genetic Transformation of Willow and Cottonwood for Increased Remediation of Pollutants
R829479C029 Development of Tightly Regulated Ecdysone Receptor-Based Gene Switches for Use in Agriculture
R829479C030 Engineered Plant Virus Proteins for Biotechnology

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.