Grantee Research Project Results

2000 Progress Report: The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields

EPA Grant Number: R827015C008
Subproject: this is subproject number 008 , established and managed by the Center Director under grant R827015
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Northeast HSRC
Center Director: Sidhu, Sukh S.
Title: The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
Investigators: Suflita, Joseph , Davidova, Irene A.
Institution: University of Oklahoma
EPA Project Officer: Aja, Hayley
Project Period: July 1, 1999 through June 30, 2000 (Extended to June 30, 2001)
Project Period Covered by this Report: July 1, 1999 through June 30, 2000
RFA: Integrated Petroleum Environmental Consortium (IPEC) (1999) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Targeted Research

Objective:

In this project we investigated, how relevant is the use of nitrate as a preferred electron acceptor to control the metabolic activity of sulfate reducing bacteria to selected oil fields in Oklahoma.

Progress Summary:

We were planning to proceed with fields trial during the fall, but the period of heavy rains did not permit us to do so. Severe winter conditions did not improve the situation and we were practically unable to start field experiments. However, we continue to monitor the rates of sulfate reduction, potential nitrate reduction and cell numbers of sulfate-reducing bacteria (SRB) and nitrate reducing bacteria (NRB). In the samplers taken in wintertime the rates were lower, than in September but all tendencies were the same (Table 1). The rate of sulfate reduction in the oil-water separator was one order of magnitude higher than in the wellhead samples. Also, in oil-water separator SRB again exceeded NRB in two orders of magnitude. The existing pattern argued that major sulfate reduction occurred in aboveground facilities.

Table 1.

Sampling Site	Sulfate Reducing Activity (µM S/d)	Potential Nitrate Reducing Activity (mmol/L/d)	NRB (cell/ml)	SRB (cell/ml)
Well 1	0.077	0.034	2.5 x 10²	2.5 x 10³
Well 2	0.034	0	2.5 x 10⁵	4.5 x 10
Oil/Water separator	0.988	0.02	15	2.5 x 10³

Journal Articles on this Report : 1 Displayed | Download in RIS Format

Publications Views
Other subproject views:	All 1 publications	1 publications in selected types	All 1 journal articles
Other center views:	All 120 publications	19 publications in selected types	All 16 journal articles

Publications
Type	Citation	Sub Project	Document Sources
Journal Article	Davidova I, Hicks MS, Fedorak PM, Suflita JM. The influence of nitrate on microbial processes in oil industry production waters. Journal of Industrial Microbiology and Biotechnology 2001;27(2):80-86.	R827015C008 (2000) R827015C008 (Final)	Abstract from PubMed Abstract: Springer Exit

Supplemental Keywords:

Sulfate reduction, nitrate reducing bacteria, microbial processes, biogeochemical, oil-water separator, oxidation, electron acceptor., RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Waste, Water, Geographic Area, Contaminated Sediments, Remediation, Chemistry, State, Civil/Environmental Engineering, Hazardous Waste, Oil Spills, Bioremediation, Engineering, Environmental Engineering, Hazardous, dentrification, microbial degradation, sulfate reducing bacteria, sulfate reducing bacterium, contaminated sediment, sulfide formation, contaminated soil, oil spill, soils, nitrate compounds, soil, treatment, contaminants in soil, bioremediation of soils, Hydrogen sulfide, hazardous waste cleanup, nitrate, Oklahoma (OK), dentrifying bacteria, leachate, bacterial degradation

Progress and Final Reports:

Original Abstract

Final Report

Main Center Abstract and Reports:

R827015 HSRC (1989) - Northeast HSRC

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827015C001 Evaluation of Road Base Material Derived from Tank Bottom Sludges
R827015C002 Passive Sampling Devices (PSDs) for Bioavailability Screening of Soils Containing Petrochemicals
R827015C003 Demonstration of a Subsurface Drainage System for the Remediation of Brine-Impacted Soil
R827015C004 Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C005 Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
R827015C006 Microbial Treatment of Naturally Occurring Radioactive Material (NORM)
R827015C007 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C008 The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
R827015C009 Surfactant-Enhanced Treatment of Oil-Contaminated Soils and Oil-Based Drill Cuttings
R827015C010 Novel Materials for Facile Separation of Petroleum Products from Aqueous Mixtures Via Magnetic Filtration
R827015C011 Development of Relevant Ecological Screening Criteria (RESC) for Petroleum Hydrocarbon-Contaminated Exploration and Production Sites
R827015C012 Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C013 New Process for Plugging Abandoned Wells
R827015C014 Enhancement of Microbial Sulfate Reduction for the Remediation of Hydrocarbon Contaminated Aquifers - A Laboratory and Field Scale Demonstration
R827015C015 Locating Oil-Water Interfaces in Process Vessels
R827015C016 Remediation of Brine Spills with Hay
R827015C017 Continuation of an Investigation into the Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C018 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C019 Biodegradation of Petroleum Hydrocarbons in Salt-Impacted Soil by Native Halophiles or Halotolerants and Strategies for Enhanced Degradation
R827015C020 Anaerobic Intrinsic Bioremediation of MTBE
R827015C021 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R827015C022 A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C023 Data for Design of Vapor Recovery Units for Crude Oil Stock Tank Emissions
R827015C024 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells
R827015C025 A Continuation of Remediation of Brine Spills with Hay
R827015C026 Identifying the Signature of the Natural Attenuation of MTBE in Goundwater Using Molecular Methods and "Bug Traps"
R827015C027 Identifying the Signature of Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and "Bug Traps"
R827015C028 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R827015C030 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R827015C031 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R827015C032 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633 Integrated Petroleum Environmental Consortium (IPEC)
R830633C001 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells (Phase II)
R830633C002 A Continuation of Remediation of Brine Spills with Hay
R830633C003 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R830633C004 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R830633C005 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633C006 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R830633C007 Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
R830633C008 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R830633C009 Use of Earthworms to Accelerate the Restoration of Oil and Brine Impacted Sites

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.