Grantee Research Project Results
1998 Progress Report: Quantitation of Heavy Metals by Immunoassay
EPA Grant Number: R824029Title: Quantitation of Heavy Metals by Immunoassay
Investigators: Blake, Diane A.
Institution: Tufts University
EPA Project Officer: Packard, Benjamin H
Project Period: September 1, 1995 through August 31, 1998
Project Period Covered by this Report: September 1, 1997 through August 31, 1998
Project Amount: $381,920
RFA: Exploratory Research - Chemistry and Physics of Water (1995) RFA Text | Recipients Lists
Research Category: Water , Safer Chemicals , Land and Waste Management
Objective:
The specific aims of this 3-year project were as follows:- To construct and optimize an immunoassay for cadmium with respect to sensitivity, precision, and interfering substances;
- To prepare new metal-specific antibodies directed towards EDTA complexes of copper, lead, and/or zinc;
- To prepare new metal-specific antibodies directed towards metals complexed with other chelating agents (DTPA and/or DOTA).
Progress Summary:
An immunoassay was developed that accurately measured ionic cadmium in ambient water samples at concentrations from 7 to 500 ppb using an antibody isolated and characterized during the previous years of funding. A paper describing this immunoassay was published in Environmental Science and Technology. A book chapter that delineates the methods required to perform this assay as a field protocol was published in Current Protocols in Field Analytical Chemistry.Two additional antibodies to metal-chelate complexes have also been isolated and characterized. An antibody directed towards chelated complexes of Pb(II) bound with high affinity and specificity to Pb(II) complexed to a cyclohexyl derivative of DTPA. It also bound with lower affinity and specificity to Pb(II) complexes of DTPA. This anti-Pb(II) antibody was used to construct 3 different prototype immunoassays for ionic lead with different limits of detection (10-200 ppm, 1-20 ppm, and 0.2 to 2 ppb). A second antibody with specificity for DTPA complexes of Ni(II) and Zn(II) has also been isolated. Its binding properties have been characterized, but no prototype assays have been developed. A manuscript reviewing our work on metal ion immunoassays was requested from the editors of Analytica Chimica Acta. This paper is in press and will be out later in 1998.
Future Activities:
Funding for this project was not renewed. The PI has requested a 6 month unfunded extension to complete manuscripts related to these studies and to prepare the hybridomas and monoclonal antibodies generated during the project for long-term storage.Journal Articles on this Report : 2 Displayed | Download in RIS Format
| Other project views: | All 11 publications | 5 publications in selected types | All 3 journal articles |
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Blake DA, Blake RC, Khosraviani M, Pavlov AR. Immunoassays for metal ions. Analytica Chimica Acta, December 1998;376(1):13-19. |
R824029 (1998) R824029 (Final) |
not available |
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Khosraviani M, Pavlov AR, Flowers GC, Blake DA. Detection of heavy metals by immunoassay: optimization and validation of a rapid, portable assay for ionic cadmium. Environmental Science & Technology 1998;32(1):137-142. |
R824029 (1998) R824029 (Final) |
not available |
Supplemental Keywords:
cadmium, lead, nickel, zinc, heavy metals, immunoassays., Scientific Discipline, Water, Toxics, National Recommended Water Quality, Mercury, Chemistry, Environmental Chemistry, Engineering, Chemistry, & Physics, Hydrology, Physics, heavy metals, metal contaminated sediment, metal ions, monoclonal antibodies, soil contaminants, lead, copper, bifunctional derivatives, cadmium, remediation, ambient emissions, immunoassay, metal speciation, Zinc, immunochemical-based detectionProgress and Final Reports:
Original AbstractThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.