Grantee Research Project Results
Final Report: Bioinspired Design and Directed Evolution of Iron Containing Enzymes for Green Synthetic Processes and Bioremediation
EPA Grant Number: SU833912Title: Bioinspired Design and Directed Evolution of Iron Containing Enzymes for Green Synthetic Processes and Bioremediation
Investigators: Solomon, Edward I. , Bell, III, Caleb B. , Wong, Shaun D. , Liu, Lei
Institution: Stanford University
EPA Project Officer: Page, Angela
Phase: I
Project Period: August 15, 2008 through August 14, 2009
Project Amount: $10,000
RFA: P3 Awards: A National Student Design Competition for Sustainability Focusing on People, Prosperity and the Planet (2008) RFA Text | Recipients Lists
Research Category: P3 Challenge Area - Air Quality , P3 Challenge Area - Chemical Safety , P3 Challenge Area - Safe and Sustainable Water Resources , Pollution Prevention/Sustainable Development , P3 Awards , Sustainable and Healthy Communities
Objective:
Enzymes are nature’s catalysts for performing highly efficient and specific chemical transformations typically resulting in low levels of pollutive byproducts; they can be used for bioremediation, conversion of chemical energy, and production of medicinal agents in an environmentally-friendly manner. We propose to develop a mechanistic understanding of intermediates employed by biological systems that will serve as a basis for synthetic design or directed evolution to generate green synthetic processes and agents of bioremediation. The challenge of creating catalysts that match the efficiency of nature and are economically viable, while contributing a minimal amount of pollution to the environment requires insight into how these enzymes work. Three iron-containing enzyme systems, methane monooxygenase (MMO), benzoate 1,2-dihydrodiol oxygenase (BZDOS) and the halogenase SyrB2, catalyze environmentally- or commercially-important processes and the insight obtained of their reaction mechanisms will be used as templates for bioinspired design and directed evolution. We have initiated spectroscopic and computational studies on these species and related model complexes. The results from these show that the nuclear resonance vibrational scattering (NRVS) method (vide infra) is highly sensitive to the geometric structure of high-valent and hydroperoxo intermediates.
Journal Articles on this Report : 1 Displayed | Download in RIS Format
Other project views: | All 1 publications | 1 publications in selected types | All 1 journal articles |
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LIu L, Bell C, Wong S, Solomon E. Definition of the intermediates and mechanism of the anticancer drug bleomycin using nuclear resonance vibrationalpectroscopy and related methods. BIOLOGICAL SCIENCES 2010;107(52):22419-22424. |
SU833912 (Final) |
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