Grantee Research Project Results
Genomic Database for Cryptosporidium spp.
EPA Grant Number: R825148Title: Genomic Database for Cryptosporidium spp.
Investigators: Upton, Steve J.
Current Investigators: Upton, Steve J. , Dykstra, Christine C. , Blagburn, Byron L.
Institution: Kansas State University
Current Institution: Kansas State University , Auburn University Main Campus
EPA Project Officer: Packard, Benjamin H
Project Period: September 23, 1996 through September 22, 1999 (Extended to September 22, 2000)
Project Amount: $304,755
RFA: Drinking Water (1996) RFA Text | Recipients Lists
Research Category: Water
Description:
Using molecular techniques, we plan to construct gDNA libraries for various isolates and species of Cryptosporidium. In total, about 12 isolates of C. parvum and perhaps 8-10 other species of the genus will be included. These libraries will be made accessible through the American Type Culture Collection for general distribution to researchers. These libraries should be useful to investigators who plan to design genetic probes for environmental testing. The advantage of these libraries will be to: 1) allow investigators to sequence and study any region of the genome of their choice without having to rely on previously published sequences from other researchers; 2) allow investigators to develop specific, rather than random, primers for developing PCR based diagnostic tests; and 3) to archive the DNA of various isolates and species in a manner where information can be retrieved in perpetuity.
Publications and Presentations:
Publications have been submitted on this project: View all 13 publications for this projectJournal Articles:
Journal Articles have been submitted on this project: View all 9 journal articles for this projectSupplemental Keywords:
pathogens, drinking water, genetics, Kansas, human health, environmental testing, exposure., RFA, Scientific Discipline, Water, Health Risk Assessment, Environmental Chemistry, Analytical Chemistry, Drinking Water, Genetics, emerging pathogens, genomic database, Safe Drinking Water, treatment, microbial risk assessment, microbial risk management, monitoring, pathogens, analytical methods, DNA libraries, drinking water system, drinking water contaminants, exposure, infectivity, human health effects, public water systems, cryptosporidium , public health, detectionRelevant Websites:
Cryptosporidium and Cryptosporidiosis Exit
Commercial reagents for Cryptosporidium research Exit
Cryptosporidium oocyst measurements Exit
Taxonomic chronology of Cryptosporidium Exit
Waterborne/foodborne outbreaks of Cryptosporidium parvum Exit
Progress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.