Grantee Research Project Results
Rapid and Quantitative Detection of Helicobacter Pylori and E. Coli O157 in Well Water Using a Nano-Wired Biosensor and QPCR
EPA Grant Number: R833005Title: Rapid and Quantitative Detection of Helicobacter Pylori and E. Coli O157 in Well Water Using a Nano-Wired Biosensor and QPCR
Investigators: Alocilja, Evangelyn C. , Rose, Joan B. , Molloy, Stephanie L.
Current Investigators: Alocilja, Evangelyn C. , Rose, Joan B. , Dreelin, Erin
Institution: Michigan State University
EPA Project Officer: Packard, Benjamin H
Project Period: November 1, 2006 through October 31, 2009 (Extended to October 31, 2011)
Project Amount: $600,000
RFA: Development and Evaluation of Innovative Approaches for the Quantitative Assessment of Pathogens in Drinking Water (2005) RFA Text | Recipients Lists
Research Category: Drinking Water , Water
Objective:
The hypothesis of this proposal is that a disposable biosensor and QPCR can be combined seamlessly to develop a unique biosensor-QPCR as a tool for near real-time determination of contaminant occurrence in drinking water. The specific objectives are: (1) Develop a protocol for processing water samples for the biosensor and QPCR; (2) Assess the performance of the biosensor and QPCR for sensitivity, specificity, recovery, and false positives/negatives of detection and enumeration for E. coli O157:H7 and H. pylori in groundwater samples from the field; (3) Develop a method for detecting and enumerating E. coli O157:H7 and H. pylori by QPCR using bacteria isolated and screened by the biosensor system; and (4) Validate a method for testing viability of E. coli O157:H7.
Approach:
The first step in our experimental approach is to evaluate the performance of the biosensor and QPCR separately. Once confirmed, a method to seamlessly integrate these two devices into a biosensor-to-QPCR field-lab technique, coupled with a viability test, will be developed.
Expected Results:
This project is expected to advance the use of antibody-based methods and molecular techniques for application to drinking water supplies. The expected deliverables are: (a) proof of concept and assessment of biosensor and QPCR techniques for recovery, detection and quantitation of bacteria in water; and (b) the development of a unique attribute that is not in the literature at the moment: the capability for direct confirmation through QPCR of presumptive results from the biosensor, thereby minimizing false positive and false negative analysis.
Publications and Presentations:
Publications have been submitted on this project: View all 11 publications for this projectJournal Articles:
Journal Articles have been submitted on this project: View all 3 journal articles for this projectSupplemental Keywords:
Field-based detection, biosensor-QPCR technique, drinking water, pathogens, innovative technology,, Sustainable Industry/Business, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, TREATMENT/CONTROL, Water, POLLUTANTS/TOXICS, Technology for Sustainable Environment, Sustainable Environment, Microorganisms, Drinking Water, Environmental Engineering, Environmental Monitoring, Technology, biosensors, E. Coli, Helicobacter pylori, biotechnology, biosensor, pathogens, nanotechnology, drinking water monitoringProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.