Grantee Research Project Results
Development of Pathway Selective Cells and Assays for Sensitive Detection of Environmental Toxicants via High Throughput Label-Free Cell-Based Screening
EPA Contract Number: EPD13015Title: Development of Pathway Selective Cells and Assays for Sensitive Detection of Environmental Toxicants via High Throughput Label-Free Cell-Based Screening
Investigators: Irlan, Jeff
Small Business: ACEA Biosciences, Inc.
EPA Contact: Richards, April
Phase: I
Project Period: May 15, 2013 through November 14, 2013
Project Amount: $78,881
RFA: Small Business Innovation Research (SBIR) - Phase I (2013) RFA Text | Recipients Lists
Research Category: Small Business Innovation Research (SBIR) , SBIR - Innovation in Manufacturing
Description:
This project will help us to research and develop for eventual commercialization a panel of validated cell lines, protocols and data analysis methods to assess toxic effects of molecules on the critical developmental pathways outlined above on existing, commercially available high-throughput label-free screening systems. The first phase consists of initial feasibility studies using receptor tyrosine kinase pathways to broadly assess which pathways can potentially be interrogated, and for each, what toxicant classes may be detected. The optimization of product design and production techniques for stable scale-up will be conducted, followed by assay optimization for each pathway-specific product. Finally, assay miniaturization, testing under HTS automation workflows, development and validation of data analysis parameters, and preliminary validation of assay selectivity will be conducted to generate a product suitable of commercialization.
Supplemental Keywords:
toxicants, tyrosine kinases, cell-based screeningProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.