Grantee Research Project Results
Final Report: Development of Pathway Selective Cells and Assays for Sensitive Detection of Environmental Toxicants via High Throughput Label-Free Cell-Based Screening
EPA Contract Number: EPD13015Title: Development of Pathway Selective Cells and Assays for Sensitive Detection of Environmental Toxicants via High Throughput Label-Free Cell-Based Screening
Investigators: Irlan, Jeff
Small Business: ACEA Biosciences, Inc.
EPA Contact: Richards, April
Phase: I
Project Period: May 15, 2013 through November 14, 2013
Project Amount: $78,881
RFA: Small Business Innovation Research (SBIR) - Phase I (2013) RFA Text | Recipients Lists
Research Category: Small Business Innovation Research (SBIR) , SBIR - Innovation in Manufacturing
Description:
Environmental pollutants that impact critical biological pathways involved in human development and physiology can have unpredictable effects on health. The project goal is to develop and evaluate to the proof-of-concept level a product for quantitative evaluation of toxic effects of molecules on developmental pathways in a cellular model system. The product concept consists of a panel of validated cell lines, protocols and data analysis methods to assess toxic effects of molecules on critical receptor mediated pathways using high-throughput, label-free screening systems.
Summary/Accomplishments (Outputs/Outcomes):
The cells were seeded onto plates containing microelectrode biosensors, resulting in alteration of the electrical impedance observed when an electric field is applied to the system. The resulting impedance readout allowed for rapid and precise measurement of subtle changes in cellular morphology or attachment, as well as long-term proliferation and cytotoxicity in response to treatment. The project successfully established a proof-of-concept for the product design.
Conclusions:
Supplemental Keywords:
toxicants, tyrosine kinases, cell-based screeningThe perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.