Science Inventory

Refine Your Search

Search Again

or try advanced search

Search Within

Search Results

Search Criteria:

Records with

  • Keyword = endocrine disruption.
  (Sorted by Fiscal Year)
Records 1 to 25 of 419

Evaluation of Alternative Estrogen Activity Assays for New Computational Models to Support the Endocrine Disruption Screening Program
(PRESENTATION) [Presented : Mar 14, 2024]
Background and Purpose The Endocrine Disruption Screening Program (EDSP) was launched by EPA to screen pesticides and other chemicals for the potential to perturb the estrogen, androgen, and thyroid pathways in humans and wildlife. Recently, EPA has worked to develop in vitro hig...
High-throughput Identification of Androgen Disrupting ToxCast Chemicals and CYP450 Metabolites
(PRESENTATION) [Presented : Mar 14, 2024]
Background and Purpose The identification of chemicals with the potential to disrupt androgen signaling is a key component of the EPA’s Endocrine Disruption Screening Program (EDSP). EPA researchers have worked to devise in vitro assays and computational models to serve as ...
Putative adverse outcome pathway development based on physiological responses of female fathead minnows to model estrogen versus androgen receptor agonists
(JOURNAL) [Published : Jun 22, 2023]
Several adverse outcome pathways (AOPs) have linked molecular initiating events like aromatase inhibition, androgen receptor (AR) agonism, and estrogen receptor (ER) antagonism to reproductive impairment in adult fish. Estrogen receptor agonists can also cause adverse reproductiv...
Verification of In Vivo Estrogenic Activity for Four Per- and Polyfluoroalkyl Substances (PFAS) Identified as Estrogen Receptor Agonists via New Approach Methodologies
(JOURNAL) [Published : Mar 07, 2023]
Given concerns about potential toxicological hazards of the thousands of data-poor per- and polyfluorinated alkyl substances (PFAS) currently in commerce and detected in the environment, tiered testing strategies that employ high-throughput in vitro screening as an initial testin...
Assessing the Predictive Value of Thyroid In Vitro Screening Assays Through Comparisons to Observed Impacts In Vivo
(PRESENTATION) [Presented : Nov 16, 2023]
Product description/abstract: Rusty will copy/paste this in from your abstract/paper. The U.S. EPA’s Endocrine Disruptor Screening Program (EDSP) was developed to investigate the potential for chemicals to interact with the vertebrate endocrine system and to characterize a...
Deiodinase inhibition impairs the formation of the three posterior swim bladder tissue layers during early embryonic development in zebrafish
(JOURNAL) [Published : Jul 12, 2023]
Thyroid hormone system disruption (THSD) negatively affects multiple developmental processes and organs. In fish, inhibition of deiodinases, which are enzymes crucial for (in)activating thyroid hormones (THs), leads to impaired swim bladder inflation. Until now, the underlying me...
Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase
(JOURNAL) [Published : Jun 26, 2022]
The transition to include in vitro-based data in chemical hazard assessment has resulted in the development and implementation of screening assays to cover a diversity of biological pathways, including recently added assays to interrogate chemical disruption of proteins relevant ...
Assessing utility of thyroid in vitro screening assays through comparisons to observed impacts in vivo
(JOURNAL) [Published : Oct 01, 2023]
To better understand endocrine disruption, the US Environmental Protection Agency's (USEPA) Endocrine Disruptor Screening Program (EDSP) utilizes a two-tiered approach to investigate the potential of a chemical to interact with the estrogen, androgen, or thyroid systems. As&...
Case Study in 21st-Century Ecotoxicology: Using In Vitro Aromatase Inhibition Data to Predict Reproductive Outcomes in Fish In Vivo
(JOURNAL) [Published : Dec 23, 2022]
To reduce the use of intact animals for chemical safety testing, while ensuring protection of ecosystems and human health, there is a demand for new approach methodologies (NAMs) that provide relevant scientific information at a quality equivalent to or better than traditional ap...
Identification of Biotransformation Products (BPs) in Rats Exposed to 6:1 Fluorotelomer Alcohol (6:1 FTOH)
(PRESENTATION) [Presented : Nov 16, 2023]
Fluorotelomer alcohols (FTOHs) are precursors and intermediates to the toxic, persistent, and bioaccumulative perfluorocarboxylic acids and are of concern to human health and the environment.  To better understand the toxicity and risk from FTOHs, male and female rats were o...
In vivo effects of four per- and polyfluoroalkyl substances identified as estrogenic via in vitro screening.
(PRESENTATION) [Presented : Mar 23, 2023]
Per- and polyfluroalkyl substances (PFAS) are a large group of chemicals of concern based on their potential persistence, bioaccumulation, and toxicity. A tiered approach to screening and testing using new approach methodologies has been proposed as one of the few tractable appro...
Identifying Thyroid-Active Chemicals Using High-Throughput Screening
(PRESENTATION) [Presented : Mar 23, 2023]
High-throughput screening (HTS) assays allow for rapidly testing many chemicals for bioactivity at specific molecular targets. One goal of HTS is to reduce the time and cost of generating data to support evaluation of chemicals for potential endocrine disruption. Data from these ...
Presentation (1): Technical Evaluation and Standardization of the Human Thyroid Microtissue Assay
(PRESENTATION) [Presented : Sep 26, 2023]
A presentation at the U.S. Environmental Protection Agency – Japan Ministry of the Environment 16th Bilateral Meeting on Endocrine Disruption Test Methods Development. The topic covers EPA activities related to New Approach Methods for endocrine disruption hazard screening. 
Reducing Uncertainties in Quantitative Adverse Outcome Pathways by Analysis of Thyroid Hormone in the Neonatal Rat Brain
(JOURNAL) [Published : Jun 01, 2023]
A number of xenobiotics interfere with thyroid hormone (TH) signaling. Although adequate supplies of TH are necessary for normal brain development, regulatory reliance on serum TH as proxies for brain TH insufficiency is fraught with significant uncertainties. A more direct causa...
Multi-omic Responses of Fish Exposed to Complex Chemical Mixtures in the Shenandoah River Watershed
(JOURNAL) [Published : Dec 01, 2023]
To evaluate relationships between different anthropogenic impacts, contaminant occurrence, and fish health, we conducted in situ fish exposures across the Shenandoah River watershed at five sites with different land use. Exposure water was analyzed for over 500 chemical constitue...
Systematic Analysis of High-Throughput Transcriptomics to Identify Potential Carcinogens
(PRESENTATION) [Presented : Mar 23, 2023]
Recent technological advances have moved the field of toxicogenomics from reliance on microarray platforms to high-throughput transcriptomic technologies that measure global gene expression. Gene expression biomarkers are emerging as useful tools for interpreting gene expression ...
High-throughput AR Dimerization Assay Identifies Androgen Disrupting Chemicals and Metabolites
(JOURNAL) [Published : Apr 04, 2023]
Introduction: Analysis of streamlined computational models used to predict androgen disrupting chemicals revealed that assays measuring androgen receptor (AR) cofactor recruitment/dimerization were particularly indispensable to high predictivity, especially for AR antagonist...
In Vitro Screening of 149 PFAS Chemicals for Potential Inhibition of the Sodium Iodide Symporter (NIS)#
(PRESENTATION) [Presented : Mar 22, 2023]
There is increased concern for environmental chemicals that can target various sites within the hypothalamic-pituitary-thyroid axis to disrupt thyroid synthesis, transport, metabolism and/or function.  One well known thyroid target that has been described in both humans and ...
New Approach Methodologies for the Endocrine Activity Toolbox: Environmental Assessment for Fish and Amphibians
(JOURNAL) [Published : Mar 29, 2023]
Multiple in vivo test guidelines focusing on the estrogen, androgen, thyroid, and steroidogenesis pathways have been developed and validated for mammals, amphibians, or fish. However, these tests are resource-intensive and often use a large number of laboratory animals. Developin...
Integrating In Silico And In Vitro Approaches to Understand Cross-Species Predictions of Chemical Susceptibility for Aromatase
(PRESENTATION) [Presented : Mar 23, 2023]
Endocrine active chemicals are of concern for chemical hazard evaluation, and several new approach methodologies (NAMs) have been developed to rapidly screen chemicals for biological activities leading to endocrine disruption. Aromatase (CYP19A1), for example, catalyzes the biosy...
Assessing ecological effects and relevance of estrogenic per- and polyfluoroalkyl substances (PFAS)
(PRESENTATION) [Presented : Apr 05, 2023]
Per- and polyfluoroalkyl substances (PFAS) are a large group of chemicals of concern due to their widespread use, accumulation in humans and wildlife, and potential for toxicity. Based on bioactivity screening, over 25% of PFAS evaluated showed some degree of estrogenic activity....
Applying EcoToxChips to Identify Potential Gene Expression Markers of Exposure to Glucocorticoid Receptor Agonists
(PRESENTATION) [Presented : Nov 17, 2022]
Glucocorticoid-mediated bioactivity has been detected in North American surface waters.  However, the bioavailability and in vivo potency of glucocorticoid receptor (GR) agonists in environmental mixtures remains undefined. To better understand the sensitivity of in vivo, he...
Current Understanding of PFAS Toxicology with Animal Models and Corresponding Findings in Epidemiological Surveys - DIOXIN 2021
(PRESENTATION) [Presented : Aug 24, 2021]
Current Understanding of PFAS Toxicology with Animal Models and Corresponding Findings in Epidemiological Surveys Christopher Lau, Center for Public Health and Environmental Assessment, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Pa...
In vitro transcriptomic analyses reveal pathway perturbations, estrogenic activities, and potencies of data-poor BPA alternative chemicals
(JOURNAL) [Published : Feb 01, 2023]
Since initial regulatory action in 2010 in Canada, bisphenol A (BPA) has been progressively replaced by structurally related alternative chemicals. Unfortunately, many of these chemicals are data-poor, limiting toxicological risk assessment. We used high-throughput transcriptomic...
Gestational Exposure To Oxyfluorfen, A Newly-Identified Thyroid Disruptor: Effects In Pregnant Rats And Their Progeny.
(PRESENTATION) [Presented : Oct 18, 2022]
Many environmental contaminants reduce circulating levels of thyroid hormones (TH). EPA has developed a suite of high-throughput in vitro assays to identify potential target sites within the thyroid axis. One important target site is the sodium iodine symporter (NIS), a transport...