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TECHNOLOGIES FOR MONITORING AND MEASUREMENT OF DIUOXIN AND DIOXIN-LIKE COMPOUNDS IN SOIL AND SEDIMENT. ABRAXIS LLC COPLANAR PCB ELISA KIT
Citation:
DINDAL, A. AND S. BILLETS. TECHNOLOGIES FOR MONITORING AND MEASUREMENT OF DIUOXIN AND DIOXIN-LIKE COMPOUNDS IN SOIL AND SEDIMENT. ABRAXIS LLC COPLANAR PCB ELISA KIT. U.S. Environmental Protection Agency, Washington, DC, EPA/540/R-05/003 (NTIS PB2005108388), 2005.
Impact/Purpose:
The objective of this program is to promote the acceptance and use of innovative field technologies by providing well-documented performance and cost data obtained from field demonstrations.
Description:
A demonstration of technologies for determining the presence of dioxin and dioxin-like compounds in soil and sediment was conducted under EPA's Superfund Innovative Technology Evaluation Program in Saginaw, Michigan in April 2004. This report describes the performance evaluation of the Abraxis LLC Coplanar PCB Enzyme-Linked Immunosorbent Assay (ELISA) kit. The kit is an immunoassay technique that reports the total toxicity equivalents (TEQ) of polychlorinated biphenyls (PCBs). The technology results were compared to high resolution mass spectrometry TEQ results generated using EPA Method 1668A.
Abraxis generally reported data that were higher than the reference laboratory TEQPCB values, with the exception of ultra-high level PCB samples [> 10,000 picogram/gram (pg/g) TEQ] where Abraxis reported values lower than the reference method. The technologys estimated MDL was 6 to 31 pg/g TEQPCB. Results from this demonstration suggest that the Abraxis kit could be an effective screening tool for screening sample concentrations above and below 50 pg/g TEQPCB, particularly considering that the cost ($22,668 vs. $184,449) and the time to analyze the 209 demonstration samples were significantly less than those of the reference laboratory.