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BIODEGRADATION OF FLUORANTHENE AS MONITORED USING STABLE CARBON ISOTOPES
Citation:
Trust, B. A., J. G. Mueller, R B. Coffin, AND L. A. Cifuentes. BIODEGRADATION OF FLUORANTHENE AS MONITORED USING STABLE CARBON ISOTOPES. Presented at Paper from Third International In Situ and On-Site Bioreclamation Symposium sponsored by Battelle, San Diego, CA, April 24 - 27, 1995.
Impact/Purpose:
Presentation
Description:
The measurement of stable isotope ratios of carbon (d13C values) was investigated as a viable technique to monitor the intrinsic bioremediation of polycyclic aromatic hydrocarbons (PAHs). Biometer-flask experiments were conducted in which the bacterium, Sphingomonas paucimobilis, designated EPA505, was grown on fluoranthene. During growth of EPA505 on fluoranthene, bacterial biomass, respired CO2 and dissolved organic carbon (DOC), as well as fluoranthene, were sampled over eight days. The concentrations and d13C values of each of these carbon pools were determined. The concentration of fluoranthene decreased from 12.1 | 2.0(n=2) to 3.0 | 0.9 (n=2)mg C per flask over 188 h, and CO2 increased from undetectable levels to 7.1 | 0.3 (n=4) mg C per flask. A total of 55.5% mineralization resulted. DOC concentrations remained fairly constant with time, averaging 2.2 to 3.6 mg C per flask. The d 13C value of fluoranthene remained constant over the course of the experiment, averaging -24.5 | 0.2 o/oo (n=8). Bacterial nucleic acids and respired CO2 took on d13C values similar to those of fluoranthene within 47 h, measuring -22.6 and -24.3 o/oo, respectively.