EPA Science Inventory

CHROMOSOMAL MULTIPLICITY IN BURKHOLDERIA CEPACIA

Citation:

Lessie, T. G. AND B. D. Manning. CHROMOSOMAL MULTIPLICITY IN BURKHOLDERIA CEPACIA. Presented at Biotechnology Risk Assessment Symposium, Pensacola, FL, June 06 - 08, 1995.

Description:

We have used CHEF gel electrophoresis to screen preparations of large DNA from different Burkholderia cepacia isolates for the presence of DNA species corresponding to the linearized forms of the three chromosomes of 3.4,2.5, and 0.9 Mb identified in B. cepacia strain 17616. DNA preparations from the different isolates contained between two and four linearized replicons in the size range of 1.1 to 5.8 Mb, confirming that chromosomal multiplicity is common among B. cepacia strains. Estimates of overall genome size varied between 4.6 and 8.5 Mb. We also compared the distribution of r-RNA (rrn) genes in selected isolates by determining the number and sizes of DNA fragments generated by treatment of their DNA with Ceu I, an enzyme which appears to cleave exclusively within 23S r-RNA genes. Ceu I fragment patterns of digests of DNA from strain 17616 were consistent with earlier results suggesting the presence of three rrn operons on the 3.4-Mb replicon, one on the 2.5-Mb replicon, and one on the 0.9-Mb replicon. Other B. cepacia isolates appeared to contain six sets of rrn genes.

Purpose/Objective:

Presentation

URLs/Downloads:

DUMMY FILE.PDF   (PDF,NA pp, 3 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (PRESENTATION/PAPER)
Start Date: 06/08/1995
Completion Date: 06/08/1995
Record Last Revised: 10/01/2012
Record Created: 12/08/2004
Record Released: 12/08/2004
OMB Category: Other
Record ID: 97738

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

GULF ECOLOGY DIVISION

MOLECULAR ECOLOGY BRANCH