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CLASSIFICATION OF PAH-DEGRADING BACTERIA BY PAH UTILIZATION PATTERNS AND THE COMPARISON OF METABOLIC PRODUCTS
Citation:
Ho, Y., Y. Yang, AND P H. Pritchard. CLASSIFICATION OF PAH-DEGRADING BACTERIA BY PAH UTILIZATION PATTERNS AND THE COMPARISON OF METABOLIC PRODUCTS. Presented at Eighth International IGT Symposium on Gas, Oil and Environmental Biotechnology, Colorado Springs, CO, December 11 - 13, 1995.
Impact/Purpose:
Presentation
Description:
Bacterial strains capable of using either phenanthrene, fluoranthene, or pyrene as sole carbon and energy sources were isolated from 16 different soil samples collected from the United States, Germany, and Norway. Thirty one strains were isolated on fluoranthene and the other twenty one strains were isolated on pyrene. Two other strains (CO6 and UMB), isolated on phenanthrene, are also included in this study. Co-metabolic degradation of PAHs, including fluorene, phenanthrene, anthracene, fluoranthene (FLA), pyrene (PYR), B[b]fluorene, B[a]anthracene, chrysene and B[a]pyrene were tested for each isolated using a plate screening technique. From our studies, the bacterial strains isolated on pyrene can use either pyrene or fluoranthene as sole carbon and energy source while most of the thirty one strains isolated on fluoranthene could not grow on pyrene as sole carbon source. However, these isolates are able to transform pyrene into other products co-metabolically. GC-FAME results suggest that many PYR degraders are Mycobacterium sp. and FLA degraders are Sphingomonas sp. Preliminary isolation of the pyrene metabolites produced by the fluoranthene degraders showed one major product by HPLC. This peak matches the pyrene metabolite produced by the pyrene isolates.